Vectorology and factor delivery in induced pluripotent stem cell reprogramming

Research output: Contribution to journalReview articlepeer-review

42 Scopus citations


Induced pluripotent stem cell (iPSC) reprogramming requires sustained expression of multiple reprogramming factors for a limited period of time (10-30 days). Conventional iPSC reprogramming was achieved using lentiviral or simple retroviral vectors. Retroviral reprogramming has flaws of insertional mutagenesis, uncontrolled silencing, residual expression and re-activation of transgenes, and immunogenicity. To overcome these issues, various technologies were explored, including adenoviral vectors, protein transduction, RNA transfection, minicircle DNA, excisable PiggyBac (PB) transposon, Cre-lox excision system, negative-sense RNA replicon, positive-sense RNA replicon, Epstein-Barr virus-based episomal plasmids, and repeated transfections of plasmids. This review provides summaries of the main vectorologies and factor delivery systems used in current reprogramming protocols.

Original languageEnglish
Pages (from-to)1301-1315
Number of pages15
JournalStem Cells and Development
Issue number12
StatePublished - 15 Jun 2014


Dive into the research topics of 'Vectorology and factor delivery in induced pluripotent stem cell reprogramming'. Together they form a unique fingerprint.

Cite this