TY - JOUR
T1 - Validation of the Applied Biosystems RapidHIT ID instrument and ACE GlobalFiler Express sample cartridge
AU - Cihlar, Jennifer Churchill
AU - Kapema, Kapema Bupe
AU - Budowle, Bruce
N1 - Funding Information:
This study was funded in part by Thermo Fisher Scientific (Sponsored Research Agreement RP0223).
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2022/1
Y1 - 2022/1
N2 - Rapid DNA platforms are fully automated systems capable of processing DNA from biological samples and interpreting the results in approximately 90 minutes with minimal human intervention. With a greater reliance on the system than on the analyst, validation data are especially needed to define the performance and limitations of commercially available Rapid DNA systems. Thus, validation studies of a Rapid DNA workflow consisting of the Applied Biosystems RapidHIT ID Instrument and RapidLINK software with a focus on the ACE GlobalFiler Express Sample Cartridge and reference buccal swabs were performed in accordance with Scientific Working Group on DNA Analysis Methods Validation Guidelines. These validation studies included assessments of sensitivity, contamination, concordance, reproducibility and repeatability, stability, inhibition, mixtures, sample reprocessing, precision, and first-pass success rate. Overall, the current Applied Biosystems RapidHIT ID Instrument with the ACE GlobalFiler Express sample cartridge was found to be a reliable tool for generation of STR profiles from reference-type buccal swabs.
AB - Rapid DNA platforms are fully automated systems capable of processing DNA from biological samples and interpreting the results in approximately 90 minutes with minimal human intervention. With a greater reliance on the system than on the analyst, validation data are especially needed to define the performance and limitations of commercially available Rapid DNA systems. Thus, validation studies of a Rapid DNA workflow consisting of the Applied Biosystems RapidHIT ID Instrument and RapidLINK software with a focus on the ACE GlobalFiler Express Sample Cartridge and reference buccal swabs were performed in accordance with Scientific Working Group on DNA Analysis Methods Validation Guidelines. These validation studies included assessments of sensitivity, contamination, concordance, reproducibility and repeatability, stability, inhibition, mixtures, sample reprocessing, precision, and first-pass success rate. Overall, the current Applied Biosystems RapidHIT ID Instrument with the ACE GlobalFiler Express sample cartridge was found to be a reliable tool for generation of STR profiles from reference-type buccal swabs.
KW - ACE cartridge
KW - Rapid DNA
KW - STR
KW - SWGDAM
KW - Validation
UR - http://www.scopus.com/inward/record.url?scp=85117004275&partnerID=8YFLogxK
U2 - 10.1007/s00414-021-02722-9
DO - 10.1007/s00414-021-02722-9
M3 - Article
C2 - 34643802
AN - SCOPUS:85117004275
SN - 0937-9827
VL - 136
SP - 13
EP - 41
JO - International Journal of Legal Medicine
JF - International Journal of Legal Medicine
IS - 1
ER -