TY - JOUR
T1 - Utilization of the mating scaffold protein in the evolution of a new signal transduction pathway for biofilm development
AU - Yi, Song
AU - Sahni, Nidhi
AU - Daniels, Karla J.
AU - Lu, Kevin L.
AU - Huang, Guanghua
AU - Garnaas, Adam M.
AU - Pujol, Claude
AU - Srikantha, Thyagarajan
AU - Soll, David R.
PY - 2011
Y1 - 2011
N2 - Among the hemiascomycetes, only Candida albicans must switch from the white phenotype to the opaque phenotype to mate. In the recent evolution of this transition, mating-incompetent white cells acquired a unique response to mating pheromone, resulting in the formation of a white cell biofilm that facilitates mating. All of the upstream components of the white cell response pathway so far analyzed have been shown to be derived from the ancestral pathway involved in mating, except for the mitogen-activated protein (MAP) kinase scaffold protein, which had not been identified. Here, through binding and mutational studies, it is demonstrated that in both the opaque and the white cell pheromone responses, Cst5 is the scaffold protein, supporting the evolutionary scenario proposed. Although Cst5 plays the same role in tethering the MAP kinases as Ste5 does in Saccharomyces cerevisiae, Cst5 is approximately one-third the size and has only one rather than four phosphorylation sites involved in activation and cytoplasmic relocalization.
AB - Among the hemiascomycetes, only Candida albicans must switch from the white phenotype to the opaque phenotype to mate. In the recent evolution of this transition, mating-incompetent white cells acquired a unique response to mating pheromone, resulting in the formation of a white cell biofilm that facilitates mating. All of the upstream components of the white cell response pathway so far analyzed have been shown to be derived from the ancestral pathway involved in mating, except for the mitogen-activated protein (MAP) kinase scaffold protein, which had not been identified. Here, through binding and mutational studies, it is demonstrated that in both the opaque and the white cell pheromone responses, Cst5 is the scaffold protein, supporting the evolutionary scenario proposed. Although Cst5 plays the same role in tethering the MAP kinases as Ste5 does in Saccharomyces cerevisiae, Cst5 is approximately one-third the size and has only one rather than four phosphorylation sites involved in activation and cytoplasmic relocalization.
UR - http://www.scopus.com/inward/record.url?scp=79955080374&partnerID=8YFLogxK
U2 - 10.1128/mBio.00237-10
DO - 10.1128/mBio.00237-10
M3 - Article
C2 - 21221248
AN - SCOPUS:79955080374
SN - 2150-7511
VL - 2
JO - mBio
JF - mBio
IS - 1
M1 - e00237-10
ER -