Utilization of the mating scaffold protein in the evolution of a new signal transduction pathway for biofilm development

Song Yi, Nidhi Sahni, Karla J. Daniels, Kevin L. Lu, Guanghua Huang, Adam M. Garnaas, Claude Pujol, Srikantha Thyagarajan, David R. Soll

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

Among the hemiascomycetes, only Candida albicans must switch from the white phenotype to the opaque phenotype to mate. In the recent evolution of this transition, mating-incompetent white cells acquired a unique response to mating pheromone, resulting in the formation of a white cell biofilm that facilitates mating. All of the upstream components of the white cell response pathway so far analyzed have been shown to be derived from the ancestral pathway involved in mating, except for the mitogen-activated protein (MAP) kinase scaffold protein, which had not been identified. Here, through binding and mutational studies, it is demonstrated that in both the opaque and the white cell pheromone responses, Cst5 is the scaffold protein, supporting the evolutionary scenario proposed. Although Cst5 plays the same role in tethering the MAP kinases as Ste5 does in Saccharomyces cerevisiae, Cst5 is approximately one-third the size and has only one rather than four phosphorylation sites involved in activation and cytoplasmic relocalization.

Original languageEnglish
JournalmBio
Volume2
Issue number1
DOIs
StatePublished - 1 Jan 2011

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Biofilms
Signal Transduction
Pheromones
Mitogen-Activated Protein Kinases
Phenotype
Proteins
Cellular Structures
Candida albicans
Saccharomyces cerevisiae
Phosphorylation

Cite this

Yi, Song ; Sahni, Nidhi ; Daniels, Karla J. ; Lu, Kevin L. ; Huang, Guanghua ; Garnaas, Adam M. ; Pujol, Claude ; Thyagarajan, Srikantha ; Soll, David R. / Utilization of the mating scaffold protein in the evolution of a new signal transduction pathway for biofilm development. In: mBio. 2011 ; Vol. 2, No. 1.
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abstract = "Among the hemiascomycetes, only Candida albicans must switch from the white phenotype to the opaque phenotype to mate. In the recent evolution of this transition, mating-incompetent white cells acquired a unique response to mating pheromone, resulting in the formation of a white cell biofilm that facilitates mating. All of the upstream components of the white cell response pathway so far analyzed have been shown to be derived from the ancestral pathway involved in mating, except for the mitogen-activated protein (MAP) kinase scaffold protein, which had not been identified. Here, through binding and mutational studies, it is demonstrated that in both the opaque and the white cell pheromone responses, Cst5 is the scaffold protein, supporting the evolutionary scenario proposed. Although Cst5 plays the same role in tethering the MAP kinases as Ste5 does in Saccharomyces cerevisiae, Cst5 is approximately one-third the size and has only one rather than four phosphorylation sites involved in activation and cytoplasmic relocalization.",
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Utilization of the mating scaffold protein in the evolution of a new signal transduction pathway for biofilm development. / Yi, Song; Sahni, Nidhi; Daniels, Karla J.; Lu, Kevin L.; Huang, Guanghua; Garnaas, Adam M.; Pujol, Claude; Thyagarajan, Srikantha; Soll, David R.

In: mBio, Vol. 2, No. 1, 01.01.2011.

Research output: Contribution to journalArticleResearchpeer-review

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