Using multiplex PCR amplification and typing kits for the analysis of DNA evidence in a serial killer case

Manfred N. Hochmeister, Bruce Budowle, Arthur Eisenberg, Urs V. Borer, Richard Dirnhofer

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Analysis of DNA evidence in a serial killer case was performed using the AmpliType® HLA-DQ alpha-, AmpliType® PM-, and the GenePrint(TM) STR Multiplex System PCR Amplification Kits. In addition, a sex typing procedure using the X-Y homologous gene amelogenin was carried out. DNA profiles from a single hair with attached sheath material, recovered from underneath the seat cover of the suspect's car seat were compared with DNA profiles derived from reference head hairs from a homicide victim. From the evidentiary sample only 9 ng of human DNA could be recovered. In a sample, where the quantity of DNA becomes a critical issue a powerful route is the simultaneous amplification of several loci (multiplex PCR). This is the first report where commercially available multiplex PCR amplification and typing kits have been introduced for the analysis of DNA evidence in a serial killer case and the analysis has been admitted in court.

Original languageEnglish
Pages (from-to)155-162
Number of pages8
JournalJournal of Forensic Sciences
Volume41
Issue number1
DOIs
StatePublished - 1996

Keywords

  • CSF1PO
  • D7S8
  • GC
  • GYPA
  • HBGG
  • HLA DQA1
  • LDLR
  • TH01
  • TPOX
  • amelogenin
  • criminalistics
  • deoxyribonucleic acid (DNA)
  • forensic science
  • genetic typing
  • hair
  • human identification
  • polymerase chain reaction (PCR)
  • reverse paternity
  • sexing
  • short tandem repeat (STR) loci

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