Uses of the NIST 26plex STR assay for human identity testing

Peter M. Vallone; Carolyn R. Hill; Kristen E. Lewis; Toni M. Diegoli; Michael D. Coble; John M. Butler

doi:10.1016/j.fsigss.2009.09.024

Uses of the NIST 26plex STR assay for human identity testing

Peter M. Vallone, Carolyn R. Hill, Kristen E. Lewis, Toni M. Diegoli, Michael D. Coble, John M. Butler

Research output: Contribution to journal › Article › peer-review

Abstract

Ongoing work at the U.S. National Institute of Standards and Technology has focused on the characterization of 26 autosomal STR loci for human identity testing. These 26 loci are in addition to the existing 13 U.S. core loci and those found in PowerPlex16 and Identifiler commercial STR typing kits. The amplification of the 26 loci has been optimized for degraded extracts in unique miniplex panels and also for reference samples as a single reaction 26plex assay. A study has been performed comparing genotypes obtained with the 26plex primers to those with miniplex panels for allele drop out and concordance. The forensic utility of the 26plex assay was evaluated for situations where additional loci are beneficial. The utility of this large multiplex was also tested in a case involving DNA extracted from degraded bone samples. The 26plex can serve as a low-cost assay (compared to commercially available kits) useful for both sorting comingled remains and providing additional markers for increased statistical support for samples that require "non-trio" family references for human identification.

Original language	English
Pages (from-to)	29-30
Number of pages	2
Journal	Forensic Science International: Genetics Supplement Series
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.fsigss.2009.09.024
State	Published - Jan 2009

Keywords

Additional loci
Kinship
Multiplex
PCR
Paternity
STR

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10.1016/j.fsigss.2009.09.024

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title = "Uses of the NIST 26plex STR assay for human identity testing",

abstract = "Ongoing work at the U.S. National Institute of Standards and Technology has focused on the characterization of 26 autosomal STR loci for human identity testing. These 26 loci are in addition to the existing 13 U.S. core loci and those found in PowerPlex16 and Identifiler commercial STR typing kits. The amplification of the 26 loci has been optimized for degraded extracts in unique miniplex panels and also for reference samples as a single reaction 26plex assay. A study has been performed comparing genotypes obtained with the 26plex primers to those with miniplex panels for allele drop out and concordance. The forensic utility of the 26plex assay was evaluated for situations where additional loci are beneficial. The utility of this large multiplex was also tested in a case involving DNA extracted from degraded bone samples. The 26plex can serve as a low-cost assay (compared to commercially available kits) useful for both sorting comingled remains and providing additional markers for increased statistical support for samples that require {"}non-trio{"} family references for human identification.",

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note = "Funding Information: This work was funded in part through interagency agreement 2008-DN-R-121 between the National Institute of Justice and the NIST Office of Law Enforcements. Points of view in this document are those of the authors and do not necessarily represent the official position or policies of the U.S. Department of Justice or Department of Defense. Commercial equipment, instruments, and materials are identified in order to specify experimental procedures as completely as possible. In no case does such identification imply a recommendation or endorsement by the National Institute of Standards and Technology nor does it imply that any of the materials, instruments or equipment identified are necessarily the best available for the purpose. ",

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AU - Butler, John M.

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Uses of the NIST 26plex STR assay for human identity testing

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Keywords

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