Upregulation of miR-20a and miR-106b is involved in the acquisition of malignancy of pediatric brainstem gliomas

Xuan Wang, Hongwei Zhang, Anling Zhang, Lei Han, Kun Wang, Ran Liu, Shaohua Yang, Peiyu Pu, Changhong Shen, Chunsheng Kang, Chunjiang Yu

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Brainstem glioma (BSG) is an entity which commonly occurs in pediatric patients and carries a dismal prognosis. However, the category of adult BSG has displayed considerably benign biological behavior, thus providing a unique perspective to comparatively understand the malignant features of pediatric BSG. microRNAs (miRNAs) have been associated with cancer development and progression. To identify miRNAs specifically involved in the acquisition of malignant progression of pediatric BSG, we analyzed the miRNA expression profiles in orthotopic models which could simulate the BSG heterogeneity by microarrays. Our research revealed that miR-20a and miR-106b (known to be closely related) were the two most robust upregulated miRNAs in pediatric BSG compared to adult subtype. Furthermore, the two types of human BSG tissue were utilized to verify the microarray data by qRT-PCR and in situ hybridization. The results indicated good consistency with that of the microarray method. In conclusion, our studies provide evidence that miR-20a and miR-106b may serve as putative causative involvement of malignant progression of pediatric BSG, thereby serving as likely novel targets for constraining the rapid fatal course of pediatric BSG.

Original languageEnglish
Pages (from-to)1293-1300
Number of pages8
JournalOncology Reports
Volume28
Issue number4
DOIs
StatePublished - Oct 2012

Keywords

  • Brainstem glioma
  • Pediatric tumor
  • miR-106b
  • miR-20a
  • microRNA

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