United arab emirates population data on three DNA tetrameric short tandem repeat loci: HUMTHO1, TPOX and CSF1PO - derived using multiplex polymerase chain reaction and manual typing

Riad A. Bayoumi, Lihadh I. Al-Gazali, Uzma Jaffer, Mohamed S.A. Nur-E-Kamal, Adekunle Dawodu, Abdulbari Bener, Valsamma Eapen, Bruce Budowle

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3 Scopus citations

Abstract

Allele and genotype frequencies for three tetrameric short tandem repeat (STR) loci: HUMTHO1, TPOX, and CSF1PO, were determined in a United Arab Emirates (UAE) national population sample (n = 119). The loci were amplified simultaneously and the PCR products were separated in polyacrylamide DNA sequencing gels. Detection of the DNA fragments was accomplished using silver staining. Allele designations were determined by comparison to an allelic ladder. One allele at each locus was sequenced to confirm the nature of the repeats and their number. Alleles at the HUMTHO1 and TPOX loci were distributed bimodally, while CSF1PO showed unimodal distribution. The observed heterozygosities were 76% for HUMTHO1, 64% for TPOX, and 71% for CSF1PO. No deviation from the Hardy-Weinberg expectations was observed in the genotype distribution for the loci TPOX and CSF1PO, but the HUMTHO1 locus did depart from HWE based on the likelihood ratio and the exact test. No correlation was detected between the alleles at any of the three pairs of loci. The allelic frequency data of these three loci in the UAE population sample can thus be used in human identity testing.

Original languageEnglish
Pages (from-to)1637-1640
Number of pages4
JournalELECTROPHORESIS
Volume18
Issue number9
DOIs
StatePublished - 1 Oct 1997

Keywords

  • Multiplex polymerase chain reaction
  • Short tandem repeats
  • United Arab Emirates

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