Ultrathin-layer polyacrylamide gel isoelectric focusing for the identification of hemoglobin variants

Bruce Budowle; Patricia Eberhardt

doi:10.3109/03630268609046442

Ultrathin-layer polyacrylamide gel isoelectric focusing for the identification of hemoglobin variants

Bruce Budowle, Patricia Eberhardt

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

An ultrathin-layer polyacrylamide gel isoelectric focusing method for typing hemoglobin is described. This method, which uses Pharmalyte pH 6.7-7.7 and an electrode wick distance of 5.0 cm, clearly resolves the A, F, S, C and a number of rare variant allelic products. The technique is ideal for rapid screening of a large number of fresh blood and/or bloodstained samples.

Original language	English
Pages (from-to)	161-172
Number of pages	12
Journal	Hemoglobin
Volume	10
Issue number	2
DOIs	https://doi.org/10.3109/03630268609046442
State	Published - 1 Jan 1986

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title = "Ultrathin-layer polyacrylamide gel isoelectric focusing for the identification of hemoglobin variants",

abstract = "An ultrathin-layer polyacrylamide gel isoelectric focusing method for typing hemoglobin is described. This method, which uses Pharmalyte pH 6.7-7.7 and an electrode wick distance of 5.0 cm, clearly resolves the A, F, S, C and a number of rare variant allelic products. The technique is ideal for rapid screening of a large number of fresh blood and/or bloodstained samples.",

author = "Bruce Budowle and Patricia Eberhardt",

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AU - Eberhardt, Patricia

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AB - An ultrathin-layer polyacrylamide gel isoelectric focusing method for typing hemoglobin is described. This method, which uses Pharmalyte pH 6.7-7.7 and an electrode wick distance of 5.0 cm, clearly resolves the A, F, S, C and a number of rare variant allelic products. The technique is ideal for rapid screening of a large number of fresh blood and/or bloodstained samples.

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JO - Hemoglobin

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ER -

Ultrathin-layer polyacrylamide gel isoelectric focusing for the identification of hemoglobin variants

Abstract

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