Ultrathin-layer polyacrylamide gel isoelectric focusing for the identification of hemoglobin variants

Bruce Budowle, Patricia Eberhardt

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

An ultrathin-layer polyacrylamide gel isoelectric focusing method for typing hemoglobin is described. This method, which uses Pharmalyte pH 6.7-7.7 and an electrode wick distance of 5.0 cm, clearly resolves the A, F, S, C and a number of rare variant allelic products. The technique is ideal for rapid screening of a large number of fresh blood and/or bloodstained samples.

Original languageEnglish
Pages (from-to)161-172
Number of pages12
JournalHemoglobin
Volume10
Issue number2
DOIs
StatePublished - 1 Jan 1986

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