Tyrosine Hydroxylase Inhibition in Substantia Nigra Decreases Movement Frequency

Michael Francis Salvatore, Tamara R. McInnis, Mark A. Cantu, Deana M. Apple, Brandon S. Pruett

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Reduced movement frequency or physical activity (bradykinesia) occurs with high prevalence in the elderly. However, loss of striatal tyrosine hydroxylase (TH) in aging humans, non-human primates, or rodents does not reach the ~ 80% loss threshold associated with bradykinesia onset in Parkinson’s disease. Moderate striatal dopamine (DA) loss, either following TH inhibition or decreased TH expression, may not affect movement frequency. In contrast, moderate DA or TH loss in the substantia nigra (SN), as occurs in aging, is of similar magnitude (~ 40%) to nigral TH loss at bradykinesia onset in Parkinson’s disease. In aged rats, increased TH expression and DA in SN alone increases movement frequency, suggesting aging-related TH and DA loss in the SN contributes to aging-related bradykinesia or decreased physical activity. To test this hypothesis, the SN was targeted with bilateral guide cannula in young (6 months old) rats, in a within-subjects design, to evaluate the impact of nigral TH inhibition on movement frequency and speed. The TH inhibitor, α-methyl-p-tyrosine (AMPT) reduced nigral DA (~ 40%) 45–150 min following infusion, without affecting DA in striatum, nucleus accumbens, or adjacent ventral tegmental area. Locomotor activity in the open-field was recorded up to 3 h following nigral saline or AMPT infusion in each test subject. During the period of nigra-specific DA reduction, movement frequency, but not movement speed, was significantly decreased. These results indicate that DA or TH loss in the SN, as observed in aging, contributes as a central mechanism of reduced movement frequency.

Original languageEnglish
Pages (from-to)2728-2740
Number of pages13
JournalMolecular Neurobiology
Volume56
Issue number4
DOIs
StatePublished - 1 Apr 2019

Fingerprint

Tyrosine 3-Monooxygenase
Substantia Nigra
Dopamine
Hypokinesia
Corpus Striatum
Parkinson Disease
Exercise
Ventral Tegmental Area
Nucleus Accumbens
Locomotion
Primates
Tyrosine
Rodentia

Keywords

  • Aging
  • Locomotor
  • Movement
  • Parkinson’s
  • Substantia nigra
  • Tyrosine hydroxylase

Cite this

Salvatore, Michael Francis ; McInnis, Tamara R. ; Cantu, Mark A. ; Apple, Deana M. ; Pruett, Brandon S. / Tyrosine Hydroxylase Inhibition in Substantia Nigra Decreases Movement Frequency. In: Molecular Neurobiology. 2019 ; Vol. 56, No. 4. pp. 2728-2740.
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Tyrosine Hydroxylase Inhibition in Substantia Nigra Decreases Movement Frequency. / Salvatore, Michael Francis; McInnis, Tamara R.; Cantu, Mark A.; Apple, Deana M.; Pruett, Brandon S.

In: Molecular Neurobiology, Vol. 56, No. 4, 01.04.2019, p. 2728-2740.

Research output: Contribution to journalArticleResearchpeer-review

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