Typing of the locus DYS19 from DNA derived from fingernail clippings using PCR concert™ rapid purification system

David A. Gangitano, Marisa G. Garófalo, Guillermo J. Juvenal, Bruce Budowle, Ricardo A. Padula

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

DNA extracted from the fingernails of female victims of a violent or aggressive act may assist in the identification of the male. Sometimes with the current autosomal STR loci, however, the victim's profile may mask the perpetrator's DNA profile or the perpetrator's DNA may be substantially lower in quantity than that of the victim's DNA. Thus, under these conditions, no characterization is possible. In this paper, an alternative DNA extraction procedure was employed, and the application of an STR locus residing on the Y chromosome DYS19 was typed to allow for genetic characterization of the perpetrator in such cases.

Original languageEnglish
Pages (from-to)175-177
Number of pages3
JournalJournal of Forensic Sciences
Volume47
Issue number1
DOIs
StatePublished - 2002

Keywords

  • DNA typing
  • DYS 19
  • Fingernail clippings
  • Forensic science
  • Polymerase chain reaction
  • Short tandem repeats
  • Y chromosome

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