Typing of esterase D by isoelectric focusing

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A method is described for obtaining reproducible resolution of Esterase D (EsD) phenotypes from human bloodstains by ultrathin‐layer polyacrylamide gel isoelectric focusing. By employing a pH 4.5–5.5 gradient and an electrode wick distance of 9.5 cm, all the common EsD variants were separated in 85 min. Electrofocusing of EsD took 53 and 60 min on the Cold Focus Apparatus and Ultrophor, respectively, when the distance separating the electrodes was 8.0 cm. Bloodstains up to 4 weeks old could be readily typed for EsD using this method.

Original languageEnglish
Pages (from-to)314-316
Number of pages3
JournalElectrophoresis
Volume5
Issue number5
DOIs
StatePublished - Feb 1984

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Isoelectric Focusing
Electrodes
Capillary Action
Proton-Motive Force
Phenotype
s-formylglutathione hydrolase

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title = "Typing of esterase D by isoelectric focusing",
abstract = "A method is described for obtaining reproducible resolution of Esterase D (EsD) phenotypes from human bloodstains by ultrathin‐layer polyacrylamide gel isoelectric focusing. By employing a pH 4.5–5.5 gradient and an electrode wick distance of 9.5 cm, all the common EsD variants were separated in 85 min. Electrofocusing of EsD took 53 and 60 min on the Cold Focus Apparatus and Ultrophor, respectively, when the distance separating the electrodes was 8.0 cm. Bloodstains up to 4 weeks old could be readily typed for EsD using this method.",
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Typing of esterase D by isoelectric focusing. / Budowle, Bruce.

In: Electrophoresis, Vol. 5, No. 5, 02.1984, p. 314-316.

Research output: Contribution to journalArticle

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AU - Budowle, Bruce

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AB - A method is described for obtaining reproducible resolution of Esterase D (EsD) phenotypes from human bloodstains by ultrathin‐layer polyacrylamide gel isoelectric focusing. By employing a pH 4.5–5.5 gradient and an electrode wick distance of 9.5 cm, all the common EsD variants were separated in 85 min. Electrofocusing of EsD took 53 and 60 min on the Cold Focus Apparatus and Ultrophor, respectively, when the distance separating the electrodes was 8.0 cm. Bloodstains up to 4 weeks old could be readily typed for EsD using this method.

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JO - ELECTROPHORESIS

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