Two-photon induced fluorescence of linear alkanes; A possible intrinsic lipid probe

I. Gryczynski, A. Razynska, J. R. Lakowicz

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We measured the fluorescence emission spectra and intensity decays of the linear alkane tetradecane when excited at 300 nm by two-photon excitation, The unquenched lifetime of tetradecane in neat solution is near 4.4 ns. The emission of tetradecane centered at 210 nm is collisionally quenched by oxygen, n-propanol and water. These results suggest that aliphatic groups in non-polar environments can display good fluorescence, and that the aliphatic side chains of detergents and lipids may serve as an intrinsic fluorescent probe of micelles and bilayers.

Original languageEnglish
Pages (from-to)291-295
Number of pages5
JournalBiophysical Chemistry
Issue number2-3
StatePublished - 1 Jan 1996



  • Lipid probe
  • Two-photon excitation
  • Vacuum ultraviolet

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