Abstract
We measured the fluorescence emission spectra and intensity decays of the linear alkane tetradecane when excited at 300 nm by two-photon excitation, The unquenched lifetime of tetradecane in neat solution is near 4.4 ns. The emission of tetradecane centered at 210 nm is collisionally quenched by oxygen, n-propanol and water. These results suggest that aliphatic groups in non-polar environments can display good fluorescence, and that the aliphatic side chains of detergents and lipids may serve as an intrinsic fluorescent probe of micelles and bilayers.
Original language | English |
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Pages (from-to) | 291-295 |
Number of pages | 5 |
Journal | Biophysical Chemistry |
Volume | 57 |
Issue number | 2-3 |
DOIs | |
State | Published - 1996 |
Keywords
- Lipid probe
- Two-photon excitation
- Vacuum ultraviolet