Two-photon induced fluorescence of linear alkanes; A possible intrinsic lipid probe

I. Gryczynski; A. Razynska; J. R. Lakowicz

doi:10.1016/0301-4622(95)00066-4

Two-photon induced fluorescence of linear alkanes; A possible intrinsic lipid probe

I. Gryczynski, A. Razynska, J. R. Lakowicz

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We measured the fluorescence emission spectra and intensity decays of the linear alkane tetradecane when excited at 300 nm by two-photon excitation, The unquenched lifetime of tetradecane in neat solution is near 4.4 ns. The emission of tetradecane centered at 210 nm is collisionally quenched by oxygen, n-propanol and water. These results suggest that aliphatic groups in non-polar environments can display good fluorescence, and that the aliphatic side chains of detergents and lipids may serve as an intrinsic fluorescent probe of micelles and bilayers.

Original language	English
Pages (from-to)	291-295
Number of pages	5
Journal	Biophysical Chemistry
Volume	57
Issue number	2-3
DOIs	https://doi.org/10.1016/0301-4622(95)00066-4
State	Published - 1996

Keywords

Lipid probe
Two-photon excitation
Vacuum ultraviolet

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10.1016/0301-4622(95)00066-4

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@article{0d826a44b62749fd9319b04bca40a184,

title = "Two-photon induced fluorescence of linear alkanes; A possible intrinsic lipid probe",

abstract = "We measured the fluorescence emission spectra and intensity decays of the linear alkane tetradecane when excited at 300 nm by two-photon excitation, The unquenched lifetime of tetradecane in neat solution is near 4.4 ns. The emission of tetradecane centered at 210 nm is collisionally quenched by oxygen, n-propanol and water. These results suggest that aliphatic groups in non-polar environments can display good fluorescence, and that the aliphatic side chains of detergents and lipids may serve as an intrinsic fluorescent probe of micelles and bilayers.",

keywords = "Lipid probe, Two-photon excitation, Vacuum ultraviolet",

author = "I. Gryczynski and A. Razynska and Lakowicz, {J. R.}",

note = "Funding Information: This work was supported by an NIH grant to the Center for Fluorescent Spectroscopy (NCRR RR-08 119). Joseph R. Lakowicz expresses appreciation to the University of Maryland School of Medicine and the Medical Biotechnology Center for their support.",

year = "1996",

doi = "10.1016/0301-4622(95)00066-4",

language = "English",

volume = "57",

pages = "291--295",

journal = "Biophysical Chemistry",

issn = "0301-4622",

publisher = "Elsevier",

number = "2-3",

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TY - JOUR

T1 - Two-photon induced fluorescence of linear alkanes; A possible intrinsic lipid probe

AU - Gryczynski, I.

AU - Razynska, A.

AU - Lakowicz, J. R.

N1 - Funding Information: This work was supported by an NIH grant to the Center for Fluorescent Spectroscopy (NCRR RR-08 119). Joseph R. Lakowicz expresses appreciation to the University of Maryland School of Medicine and the Medical Biotechnology Center for their support.

PY - 1996

Y1 - 1996

N2 - We measured the fluorescence emission spectra and intensity decays of the linear alkane tetradecane when excited at 300 nm by two-photon excitation, The unquenched lifetime of tetradecane in neat solution is near 4.4 ns. The emission of tetradecane centered at 210 nm is collisionally quenched by oxygen, n-propanol and water. These results suggest that aliphatic groups in non-polar environments can display good fluorescence, and that the aliphatic side chains of detergents and lipids may serve as an intrinsic fluorescent probe of micelles and bilayers.

AB - We measured the fluorescence emission spectra and intensity decays of the linear alkane tetradecane when excited at 300 nm by two-photon excitation, The unquenched lifetime of tetradecane in neat solution is near 4.4 ns. The emission of tetradecane centered at 210 nm is collisionally quenched by oxygen, n-propanol and water. These results suggest that aliphatic groups in non-polar environments can display good fluorescence, and that the aliphatic side chains of detergents and lipids may serve as an intrinsic fluorescent probe of micelles and bilayers.

KW - Lipid probe

KW - Two-photon excitation

KW - Vacuum ultraviolet

UR - http://www.scopus.com/inward/record.url?scp=0029655617&partnerID=8YFLogxK

U2 - 10.1016/0301-4622(95)00066-4

DO - 10.1016/0301-4622(95)00066-4

M3 - Article

C2 - 8573682

AN - SCOPUS:0029655617

SN - 0301-4622

VL - 57

SP - 291

EP - 295

JO - Biophysical Chemistry

JF - Biophysical Chemistry

IS - 2-3

ER -

Two-photon induced fluorescence of linear alkanes; A possible intrinsic lipid probe

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Keywords

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