Abstract
We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400 GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field.
Original language | English |
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Pages (from-to) | 78-84 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 328 |
Issue number | 1 |
DOIs | |
State | Published - 4 Mar 2005 |
Keywords
- Cy5
- Silver islands
- Two-photon excitation