Abstract
We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400 GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field.
| Original language | English |
|---|---|
| Pages (from-to) | 78-84 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 328 |
| Issue number | 1 |
| DOIs | |
| State | Published - 4 Mar 2005 |
Fingerprint
Keywords
- Cy5
- Silver islands
- Two-photon excitation
Cite this
}
Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films. / Lukomska, Joanna; Gryczynski, Ignacy; Malicka, Joanna; Makowiec, Slawomir; Lakowicz, Joseph R.; Gryczynski, Zygmunt.
In: Biochemical and Biophysical Research Communications, Vol. 328, No. 1, 04.03.2005, p. 78-84.Research output: Contribution to journal › Article
TY - JOUR
T1 - Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films
AU - Lukomska, Joanna
AU - Gryczynski, Ignacy
AU - Malicka, Joanna
AU - Makowiec, Slawomir
AU - Lakowicz, Joseph R.
AU - Gryczynski, Zygmunt
PY - 2005/3/4
Y1 - 2005/3/4
N2 - We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400 GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field.
AB - We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400 GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field.
KW - Cy5
KW - Silver islands
KW - Two-photon excitation
UR - http://www.scopus.com/inward/record.url?scp=12844256464&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2004.12.148
DO - 10.1016/j.bbrc.2004.12.148
M3 - Article
C2 - 15670753
AN - SCOPUS:12844256464
VL - 328
SP - 78
EP - 84
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -