Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films

Joanna Lukomska; Ignacy Gryczynski; Joanna Malicka; Slawomir Makowiec; Joseph R. Lakowicz; Zygmunt Gryczynski

doi:10.1016/j.bbrc.2004.12.148

Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films

Joanna Lukomska, Ignacy Gryczynski, Joanna Malicka, Slawomir Makowiec, Joseph R. Lakowicz, Zygmunt Gryczynski

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22 Scopus citations

Abstract

We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400 GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field.

Original language	English
Pages (from-to)	78-84
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	328
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2004.12.148
State	Published - 4 Mar 2005

Keywords

Cy5
Silver islands
Two-photon excitation

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10.1016/j.bbrc.2004.12.148

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Lukomska, J., Gryczynski, I., Malicka, J., Makowiec, S., Lakowicz, J. R., & Gryczynski, Z. (2005). Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films. Biochemical and Biophysical Research Communications, 328(1), 78-84. https://doi.org/10.1016/j.bbrc.2004.12.148

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title = "Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films",

abstract = "We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400 GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field.",

keywords = "Cy5, Silver islands, Two-photon excitation",

author = "Joanna Lukomska and Ignacy Gryczynski and Joanna Malicka and Slawomir Makowiec and Lakowicz, {Joseph R.} and Zygmunt Gryczynski",

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AU - Lukomska, Joanna

AU - Gryczynski, Ignacy

AU - Malicka, Joanna

AU - Makowiec, Slawomir

AU - Lakowicz, Joseph R.

AU - Gryczynski, Zygmunt

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AB - We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400 GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field.

KW - Cy5

KW - Silver islands

KW - Two-photon excitation

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