Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films

Joanna Lukomska, Ignacy Gryczynski, Joanna Malicka, Slawomir Makowiec, Joseph R. Lakowicz, Zygmunt Gryczynski

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400 GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field.

Original languageEnglish
Pages (from-to)78-84
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume328
Issue number1
DOIs
StatePublished - 4 Mar 2005

Keywords

  • Cy5
  • Silver islands
  • Two-photon excitation

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