Transplanted neural stem cells modulate regulatory T, γδ T cells and corresponding cytokines after intracerebral hemorrhage in rats

Lu Gao, Qin Lu, Li Jie Huang, Lin Hui Ruan, Jian Jing Yang, Wei Long Huang, Wei Shan ZhuGe, Yong Liang Zhang, Biao Fu, Kun Lin Jin, Qi Chuan ZhuGe

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The immune system, particularly T lymphocytes and cytokines, has been implicated in the progression of brain injury after intracerebral hemorrhage (ICH). Although studies have shown that transplanted neural stem cells (NSCs) protect the central nervous system (CNS) from inflammatory damage, their effects on subpopulations of T lymphocytes and their corresponding cytokines are largely unexplored. Here, rats were subjected to ICH and NSCs were intracerebrally injected at 3 h after ICH. The profiles of subpopulations of T cells in the brain and peripheral blood were analyzed by flow cytometry. We found that regulatory T (Treg) cells in the brain and peripheral blood were increased, but γδT cells (gamma delta T cells) were decreased, along with increased anti-inflammatory cytokines (IL-4, IL-10 and TGF-β) and decreased pro-inflammatory cytokines (IL-6, and IFN-γ), compared to the vehicle-treated control. Our data suggest that transplanted NSCs protect brain injury after ICH via modulation of Treg and γδT cell infiltration and anti- and pro-inflammatory cytokine release.

Original languageEnglish
Pages (from-to)4431-4441
Number of pages11
JournalInternational journal of molecular sciences
Volume15
Issue number3
DOIs
Publication statusPublished - 13 Mar 2014

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Keywords

  • ICH
  • Immunomodulation
  • NSCs
  • T lymphocyte subpopulations
  • Transplantation

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