Transient focal cerebral ischemia induces long-term cognitive function deficit in an experimental ischemic stroke model

Wenjun Li, Ren-Qi Huang, Ritu Anand Shetty, Nopporn Thangthaeng, Ran Liu, Zhenglan Chen, Nathalie Sumien, Margaret Rutledge, Glenn H. Dillon, Fang Yuan, Michael J. Forster, James W. Simpkins, Shaohua Yang

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Vascular dementia ranks as the second leading cause of dementia in the United States. However, its underlying pathophysiological mechanism is not fully understood and no effective treatment is available. The purpose of the current study was to evaluate long-term cognitive deficits induced by transient middle cerebral artery occlusion (tMCAO) in rats and to investigate the underlying mechanism. Sprague-Dawley rats were subjected to tMCAO or sham surgery. Behavior tests for locomotor activity and cognitive function were conducted at 7 or 30days after stroke. Hippocampal long term potentiation (LTP) and involvement of GABAergic neurotransmission were evaluated at 30days after sham surgery or stroke. Immunohistochemistry and Western blot analyses were conducted to determine the effect of tMCAO on cell signaling in the hippocampus. Transient MCAO induced a progressive deficiency in spatial performance. At 30days after stroke, no neuron loss or synaptic marker change in the hippocampus were observed. LTP in both hippocampi was reduced at 30days after stroke. This LTP impairment was prevented by blocking GABAA receptors. In addition, ERK activity was significantly reduced in both hippocampi. In summary, we identified a progressive decline in spatial learning and memory after ischemic stroke that correlates with suppression of hippocampal LTP, elevation of GABAergic neurotransmission, and inhibition of ERK activation. Our results indicate that the attenuation of GABAergic activity or enhancement of ERK/MAPK activation in the hippocampus might be potential therapeutic approaches to prevent or attenuate cognitive impairment after ischemic stroke.

Original languageEnglish
Pages (from-to)18-25
Number of pages8
JournalNeurobiology of Disease
Volume59
DOIs
StatePublished - 1 Nov 2013

Fingerprint

Transient Ischemic Attack
Cognition
Long-Term Potentiation
Stroke
Hippocampus
Middle Cerebral Artery Infarction
Synaptic Transmission
Vascular Dementia
GABA-A Receptors
Locomotion
Sprague Dawley Rats
Dementia
Western Blotting
Immunohistochemistry
Neurons
Therapeutics

Keywords

  • Cognition
  • ERK
  • GABA
  • Hippocampus
  • Long term potentiation
  • Stroke
  • Vascular dementia

Cite this

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title = "Transient focal cerebral ischemia induces long-term cognitive function deficit in an experimental ischemic stroke model",
abstract = "Vascular dementia ranks as the second leading cause of dementia in the United States. However, its underlying pathophysiological mechanism is not fully understood and no effective treatment is available. The purpose of the current study was to evaluate long-term cognitive deficits induced by transient middle cerebral artery occlusion (tMCAO) in rats and to investigate the underlying mechanism. Sprague-Dawley rats were subjected to tMCAO or sham surgery. Behavior tests for locomotor activity and cognitive function were conducted at 7 or 30days after stroke. Hippocampal long term potentiation (LTP) and involvement of GABAergic neurotransmission were evaluated at 30days after sham surgery or stroke. Immunohistochemistry and Western blot analyses were conducted to determine the effect of tMCAO on cell signaling in the hippocampus. Transient MCAO induced a progressive deficiency in spatial performance. At 30days after stroke, no neuron loss or synaptic marker change in the hippocampus were observed. LTP in both hippocampi was reduced at 30days after stroke. This LTP impairment was prevented by blocking GABAA receptors. In addition, ERK activity was significantly reduced in both hippocampi. In summary, we identified a progressive decline in spatial learning and memory after ischemic stroke that correlates with suppression of hippocampal LTP, elevation of GABAergic neurotransmission, and inhibition of ERK activation. Our results indicate that the attenuation of GABAergic activity or enhancement of ERK/MAPK activation in the hippocampus might be potential therapeutic approaches to prevent or attenuate cognitive impairment after ischemic stroke.",
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author = "Wenjun Li and Ren-Qi Huang and Shetty, {Ritu Anand} and Nopporn Thangthaeng and Ran Liu and Zhenglan Chen and Nathalie Sumien and Margaret Rutledge and Dillon, {Glenn H.} and Fang Yuan and Forster, {Michael J.} and Simpkins, {James W.} and Shaohua Yang",
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Transient focal cerebral ischemia induces long-term cognitive function deficit in an experimental ischemic stroke model. / Li, Wenjun; Huang, Ren-Qi; Shetty, Ritu Anand; Thangthaeng, Nopporn; Liu, Ran; Chen, Zhenglan; Sumien, Nathalie; Rutledge, Margaret; Dillon, Glenn H.; Yuan, Fang; Forster, Michael J.; Simpkins, James W.; Yang, Shaohua.

In: Neurobiology of Disease, Vol. 59, 01.11.2013, p. 18-25.

Research output: Contribution to journalArticle

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AU - Li, Wenjun

AU - Huang, Ren-Qi

AU - Shetty, Ritu Anand

AU - Thangthaeng, Nopporn

AU - Liu, Ran

AU - Chen, Zhenglan

AU - Sumien, Nathalie

AU - Rutledge, Margaret

AU - Dillon, Glenn H.

AU - Yuan, Fang

AU - Forster, Michael J.

AU - Simpkins, James W.

AU - Yang, Shaohua

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AB - Vascular dementia ranks as the second leading cause of dementia in the United States. However, its underlying pathophysiological mechanism is not fully understood and no effective treatment is available. The purpose of the current study was to evaluate long-term cognitive deficits induced by transient middle cerebral artery occlusion (tMCAO) in rats and to investigate the underlying mechanism. Sprague-Dawley rats were subjected to tMCAO or sham surgery. Behavior tests for locomotor activity and cognitive function were conducted at 7 or 30days after stroke. Hippocampal long term potentiation (LTP) and involvement of GABAergic neurotransmission were evaluated at 30days after sham surgery or stroke. Immunohistochemistry and Western blot analyses were conducted to determine the effect of tMCAO on cell signaling in the hippocampus. Transient MCAO induced a progressive deficiency in spatial performance. At 30days after stroke, no neuron loss or synaptic marker change in the hippocampus were observed. LTP in both hippocampi was reduced at 30days after stroke. This LTP impairment was prevented by blocking GABAA receptors. In addition, ERK activity was significantly reduced in both hippocampi. In summary, we identified a progressive decline in spatial learning and memory after ischemic stroke that correlates with suppression of hippocampal LTP, elevation of GABAergic neurotransmission, and inhibition of ERK activation. Our results indicate that the attenuation of GABAergic activity or enhancement of ERK/MAPK activation in the hippocampus might be potential therapeutic approaches to prevent or attenuate cognitive impairment after ischemic stroke.

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