Transient expression of a p58 protein kinase cDNA enhances mammalian glycosyltransferase activity

Bruce A. Bunnell, Donald E. Adams, Vincent J. Kidd

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The effect of expression of a p58 protein kinase on mammalian β-1,4 galactosyltransferase enzyme activity was examined in vitro and in vivo. We found that p58 protein kinase expression enhanced galactosyltransferase enzyme activity approximately three-fold in vivo when compared to reporter gene activity. Galactosyltransferase enzyme activity was also substantially reduced in vitro when dephosphorylated, or when p58 specific antibodies were used to inhibit kinase activity. These results suggest that galactosyltransferase activity is influenced by phosphorylation, and that the p58 protein kinase may mediate this effect.

Original languageEnglish
Pages (from-to)196-203
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume171
Issue number1
DOIs
StatePublished - 31 Aug 1990

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