Transient effects in quenching detected by harmonic-content frequency-domain fluorometry

Joseph R. Lakowicz; Michael L. Johnson; Nanda Joshi; Ignacy Gryczynski; Gabor Laczko

doi:10.1016/0009-2614(86)87163-9

Transient effects in quenching detected by harmonic-content frequency-domain fluorometry

Joseph R. Lakowicz, Michael L. Johnson, Nanda Joshi, Ignacy Gryczynski, Gabor Laczko

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3 Scopus citations

Abstract

Transient effects in the diffusional quenching of indole fluorescence by acrylamide were detected by frequency-domain fluorometry. The samples are excited with a repetitive train of picosecond pulses, and the frequency response is measured using the harmonic content up to 1 GHz. The decay of unquenched indole in aqueous buffer is dominantly a single exponential. In the presence of acrylamide the partially quenched indole fluorescence became distinctly more complex. Analysis of the frequency response of the fluorescence yields the apparent interaction radius (7.1 Å) and mutual diffusion coefficient (1.1 × 10^-5 cm² s).

Original language	English
Pages (from-to)	343-348
Number of pages	6
Journal	Chemical Physics Letters
Volume	131
Issue number	4-5
DOIs	https://doi.org/10.1016/0009-2614(86)87163-9
State	Published - 1986

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title = "Transient effects in quenching detected by harmonic-content frequency-domain fluorometry",

abstract = "Transient effects in the diffusional quenching of indole fluorescence by acrylamide were detected by frequency-domain fluorometry. The samples are excited with a repetitive train of picosecond pulses, and the frequency response is measured using the harmonic content up to 1 GHz. The decay of unquenched indole in aqueous buffer is dominantly a single exponential. In the presence of acrylamide the partially quenched indole fluorescence became distinctly more complex. Analysis of the frequency response of the fluorescence yields the apparent interaction radius (7.1 {\AA}) and mutual diffusion coefficient (1.1 × 10-5 cm2 s).",

author = "Lakowicz, {Joseph R.} and Johnson, {Michael L.} and Nanda Joshi and Ignacy Gryczynski and Gabor Laczko",

year = "1986",

doi = "10.1016/0009-2614(86)87163-9",

language = "English",

volume = "131",

pages = "343--348",

journal = "Chemical Physics Letters",

issn = "0009-2614",

publisher = "Elsevier",

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T1 - Transient effects in quenching detected by harmonic-content frequency-domain fluorometry

AU - Lakowicz, Joseph R.

AU - Johnson, Michael L.

AU - Joshi, Nanda

AU - Gryczynski, Ignacy

AU - Laczko, Gabor

PY - 1986

Y1 - 1986

N2 - Transient effects in the diffusional quenching of indole fluorescence by acrylamide were detected by frequency-domain fluorometry. The samples are excited with a repetitive train of picosecond pulses, and the frequency response is measured using the harmonic content up to 1 GHz. The decay of unquenched indole in aqueous buffer is dominantly a single exponential. In the presence of acrylamide the partially quenched indole fluorescence became distinctly more complex. Analysis of the frequency response of the fluorescence yields the apparent interaction radius (7.1 Å) and mutual diffusion coefficient (1.1 × 10-5 cm2 s).

AB - Transient effects in the diffusional quenching of indole fluorescence by acrylamide were detected by frequency-domain fluorometry. The samples are excited with a repetitive train of picosecond pulses, and the frequency response is measured using the harmonic content up to 1 GHz. The decay of unquenched indole in aqueous buffer is dominantly a single exponential. In the presence of acrylamide the partially quenched indole fluorescence became distinctly more complex. Analysis of the frequency response of the fluorescence yields the apparent interaction radius (7.1 Å) and mutual diffusion coefficient (1.1 × 10-5 cm2 s).

UR - http://www.scopus.com/inward/record.url?scp=0000828968&partnerID=8YFLogxK

U2 - 10.1016/0009-2614(86)87163-9

DO - 10.1016/0009-2614(86)87163-9

M3 - Article

AN - SCOPUS:0000828968

SN - 0009-2614

VL - 131

SP - 343

EP - 348

JO - Chemical Physics Letters

JF - Chemical Physics Letters

IS - 4-5

ER -

Transient effects in quenching detected by harmonic-content frequency-domain fluorometry

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