Transient effects in quenching detected by harmonic-content frequency-domain fluorometry

Joseph R. Lakowicz, Michael L. Johnson, Nanda Joshi, Ignacy Gryczynski, Gabor Laczko

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Transient effects in the diffusional quenching of indole fluorescence by acrylamide were detected by frequency-domain fluorometry. The samples are excited with a repetitive train of picosecond pulses, and the frequency response is measured using the harmonic content up to 1 GHz. The decay of unquenched indole in aqueous buffer is dominantly a single exponential. In the presence of acrylamide the partially quenched indole fluorescence became distinctly more complex. Analysis of the frequency response of the fluorescence yields the apparent interaction radius (7.1 Å) and mutual diffusion coefficient (1.1 × 10-5 cm2 s).

Original languageEnglish
Pages (from-to)343-348
Number of pages6
JournalChemical Physics Letters
Volume131
Issue number4-5
DOIs
StatePublished - 1 Jan 1986

Fingerprint

indoles
Quenching
Acrylamide
Fluorescence
quenching
harmonics
fluorescence
frequency response
Frequency response
picosecond pulses
Buffers
diffusion coefficient
buffers
radii
decay
indole
interactions

Cite this

Lakowicz, Joseph R. ; Johnson, Michael L. ; Joshi, Nanda ; Gryczynski, Ignacy ; Laczko, Gabor. / Transient effects in quenching detected by harmonic-content frequency-domain fluorometry. In: Chemical Physics Letters. 1986 ; Vol. 131, No. 4-5. pp. 343-348.
@article{af3a77e5ef13461488b50ecc75cf8371,
title = "Transient effects in quenching detected by harmonic-content frequency-domain fluorometry",
abstract = "Transient effects in the diffusional quenching of indole fluorescence by acrylamide were detected by frequency-domain fluorometry. The samples are excited with a repetitive train of picosecond pulses, and the frequency response is measured using the harmonic content up to 1 GHz. The decay of unquenched indole in aqueous buffer is dominantly a single exponential. In the presence of acrylamide the partially quenched indole fluorescence became distinctly more complex. Analysis of the frequency response of the fluorescence yields the apparent interaction radius (7.1 {\AA}) and mutual diffusion coefficient (1.1 × 10-5 cm2 s).",
author = "Lakowicz, {Joseph R.} and Johnson, {Michael L.} and Nanda Joshi and Ignacy Gryczynski and Gabor Laczko",
year = "1986",
month = "1",
day = "1",
doi = "10.1016/0009-2614(86)87163-9",
language = "English",
volume = "131",
pages = "343--348",
journal = "Chemical Physics Letters",
issn = "0009-2614",
publisher = "Elsevier",
number = "4-5",

}

Transient effects in quenching detected by harmonic-content frequency-domain fluorometry. / Lakowicz, Joseph R.; Johnson, Michael L.; Joshi, Nanda; Gryczynski, Ignacy; Laczko, Gabor.

In: Chemical Physics Letters, Vol. 131, No. 4-5, 01.01.1986, p. 343-348.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Transient effects in quenching detected by harmonic-content frequency-domain fluorometry

AU - Lakowicz, Joseph R.

AU - Johnson, Michael L.

AU - Joshi, Nanda

AU - Gryczynski, Ignacy

AU - Laczko, Gabor

PY - 1986/1/1

Y1 - 1986/1/1

N2 - Transient effects in the diffusional quenching of indole fluorescence by acrylamide were detected by frequency-domain fluorometry. The samples are excited with a repetitive train of picosecond pulses, and the frequency response is measured using the harmonic content up to 1 GHz. The decay of unquenched indole in aqueous buffer is dominantly a single exponential. In the presence of acrylamide the partially quenched indole fluorescence became distinctly more complex. Analysis of the frequency response of the fluorescence yields the apparent interaction radius (7.1 Å) and mutual diffusion coefficient (1.1 × 10-5 cm2 s).

AB - Transient effects in the diffusional quenching of indole fluorescence by acrylamide were detected by frequency-domain fluorometry. The samples are excited with a repetitive train of picosecond pulses, and the frequency response is measured using the harmonic content up to 1 GHz. The decay of unquenched indole in aqueous buffer is dominantly a single exponential. In the presence of acrylamide the partially quenched indole fluorescence became distinctly more complex. Analysis of the frequency response of the fluorescence yields the apparent interaction radius (7.1 Å) and mutual diffusion coefficient (1.1 × 10-5 cm2 s).

UR - http://www.scopus.com/inward/record.url?scp=0000828968&partnerID=8YFLogxK

U2 - 10.1016/0009-2614(86)87163-9

DO - 10.1016/0009-2614(86)87163-9

M3 - Article

AN - SCOPUS:0000828968

VL - 131

SP - 343

EP - 348

JO - Chemical Physics Letters

JF - Chemical Physics Letters

SN - 0009-2614

IS - 4-5

ER -