A method was described for subtyping transferrin derived from human bloodstains. Bloodstain cuttings were extracted in 0.5% ferrous ammonium sulfate. The extracts were subjected to ultrathin-layer polyacrylamide gel isoelectric focusing. After isoelectric focusing, transferrin was detected by silver staining. This method permitted the successful typing of Tf in 6-month-old blood stains maintained at -20°C and room temperature and 3-month-old bloodstains maintained at 37°C.
- Isoelectric focusing
- Ultrathin-layer polyacrylamide gel isoelectric focusing