Transferrin subtyping of human bloodstains

Brude Budowle; Elaine Scott

doi:10.1016/0379-0738(85)90136-7

Transferrin subtyping of human bloodstains

Brude Budowle, Elaine Scott

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A method was described for subtyping transferrin derived from human bloodstains. Bloodstain cuttings were extracted in 0.5% ferrous ammonium sulfate. The extracts were subjected to ultrathin-layer polyacrylamide gel isoelectric focusing. After isoelectric focusing, transferrin was detected by silver staining. This method permitted the successful typing of Tf in 6-month-old blood stains maintained at -20°C and room temperature and 3-month-old bloodstains maintained at 37°C.

Original language	English
Pages (from-to)	269-275
Number of pages	7
Journal	Forensic Science International
Volume	28
Issue number	3-4
DOIs	https://doi.org/10.1016/0379-0738(85)90136-7
State	Published - Aug 1985

Keywords

Bloodstains
Isoelectric focusing
Transferrin
Ultrathin-layer polyacrylamide gel isoelectric focusing

Access to Document

10.1016/0379-0738(85)90136-7

Cite this

@article{ccb0a3af06db49edbf9743cb7f61943b,

title = "Transferrin subtyping of human bloodstains",

abstract = "A method was described for subtyping transferrin derived from human bloodstains. Bloodstain cuttings were extracted in 0.5% ferrous ammonium sulfate. The extracts were subjected to ultrathin-layer polyacrylamide gel isoelectric focusing. After isoelectric focusing, transferrin was detected by silver staining. This method permitted the successful typing of Tf in 6-month-old blood stains maintained at -20°C and room temperature and 3-month-old bloodstains maintained at 37°C.",

keywords = "Bloodstains, Isoelectric focusing, Transferrin, Ultrathin-layer polyacrylamide gel isoelectric focusing",

author = "Brude Budowle and Elaine Scott",

year = "1985",

month = aug,

doi = "10.1016/0379-0738(85)90136-7",

language = "English",

volume = "28",

pages = "269--275",

journal = "Forensic Science International",

issn = "0379-0738",

publisher = "Elsevier Ireland Ltd",

number = "3-4",

}

TY - JOUR

T1 - Transferrin subtyping of human bloodstains

AU - Budowle, Brude

AU - Scott, Elaine

PY - 1985/8

Y1 - 1985/8

N2 - A method was described for subtyping transferrin derived from human bloodstains. Bloodstain cuttings were extracted in 0.5% ferrous ammonium sulfate. The extracts were subjected to ultrathin-layer polyacrylamide gel isoelectric focusing. After isoelectric focusing, transferrin was detected by silver staining. This method permitted the successful typing of Tf in 6-month-old blood stains maintained at -20°C and room temperature and 3-month-old bloodstains maintained at 37°C.

AB - A method was described for subtyping transferrin derived from human bloodstains. Bloodstain cuttings were extracted in 0.5% ferrous ammonium sulfate. The extracts were subjected to ultrathin-layer polyacrylamide gel isoelectric focusing. After isoelectric focusing, transferrin was detected by silver staining. This method permitted the successful typing of Tf in 6-month-old blood stains maintained at -20°C and room temperature and 3-month-old bloodstains maintained at 37°C.

KW - Bloodstains

KW - Isoelectric focusing

KW - Transferrin

KW - Ultrathin-layer polyacrylamide gel isoelectric focusing

UR - http://www.scopus.com/inward/record.url?scp=0022349580&partnerID=8YFLogxK

U2 - 10.1016/0379-0738(85)90136-7

DO - 10.1016/0379-0738(85)90136-7

M3 - Article

C2 - 4065783

AN - SCOPUS:0022349580

SN - 0379-0738

VL - 28

SP - 269

EP - 275

JO - Forensic Science International

JF - Forensic Science International

IS - 3-4

ER -

Transferrin subtyping of human bloodstains

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this