Transferrin subtypes determined by ultrathin‐layer polyacrylamide gel isoelectric focusing

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

An ultrathin‐layer polyacrylamide gel isoelectric focusing method is described for subtyping transferrin. After a one‐hour pretreatment at room temperature of transferrin in serum with ferrous ammonium sulfate, the samples were subjected to isoelectric focusing for 140 min with a final voltage of 3500 V. Thus, transferrin subtyping was achieved in one‐half of a working day. Population data on transferrin in Black and White samples from Baltimore, Maryland, were obtained using this method. The data were similar to that previously reported on these races from other geographical locations.

Original languageEnglish
Pages (from-to)97-99
Number of pages3
JournalElectrophoresis
Volume6
Issue number2
DOIs
StatePublished - 1 Jan 1985

Fingerprint

Isoelectric Focusing
Transferrin
Baltimore
Temperature
polyacrylamide gels
Electric potential
Serum
Population

Cite this

@article{efc42fbccdfd40bea957185b658be8b4,
title = "Transferrin subtypes determined by ultrathin‐layer polyacrylamide gel isoelectric focusing",
abstract = "An ultrathin‐layer polyacrylamide gel isoelectric focusing method is described for subtyping transferrin. After a one‐hour pretreatment at room temperature of transferrin in serum with ferrous ammonium sulfate, the samples were subjected to isoelectric focusing for 140 min with a final voltage of 3500 V. Thus, transferrin subtyping was achieved in one‐half of a working day. Population data on transferrin in Black and White samples from Baltimore, Maryland, were obtained using this method. The data were similar to that previously reported on these races from other geographical locations.",
author = "Bruce Budowle",
year = "1985",
month = "1",
day = "1",
doi = "10.1002/elps.1150060210",
language = "English",
volume = "6",
pages = "97--99",
journal = "ELECTROPHORESIS",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "2",

}

Transferrin subtypes determined by ultrathin‐layer polyacrylamide gel isoelectric focusing. / Budowle, Bruce.

In: Electrophoresis, Vol. 6, No. 2, 01.01.1985, p. 97-99.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Transferrin subtypes determined by ultrathin‐layer polyacrylamide gel isoelectric focusing

AU - Budowle, Bruce

PY - 1985/1/1

Y1 - 1985/1/1

N2 - An ultrathin‐layer polyacrylamide gel isoelectric focusing method is described for subtyping transferrin. After a one‐hour pretreatment at room temperature of transferrin in serum with ferrous ammonium sulfate, the samples were subjected to isoelectric focusing for 140 min with a final voltage of 3500 V. Thus, transferrin subtyping was achieved in one‐half of a working day. Population data on transferrin in Black and White samples from Baltimore, Maryland, were obtained using this method. The data were similar to that previously reported on these races from other geographical locations.

AB - An ultrathin‐layer polyacrylamide gel isoelectric focusing method is described for subtyping transferrin. After a one‐hour pretreatment at room temperature of transferrin in serum with ferrous ammonium sulfate, the samples were subjected to isoelectric focusing for 140 min with a final voltage of 3500 V. Thus, transferrin subtyping was achieved in one‐half of a working day. Population data on transferrin in Black and White samples from Baltimore, Maryland, were obtained using this method. The data were similar to that previously reported on these races from other geographical locations.

UR - http://www.scopus.com/inward/record.url?scp=0021932538&partnerID=8YFLogxK

U2 - 10.1002/elps.1150060210

DO - 10.1002/elps.1150060210

M3 - Article

AN - SCOPUS:0021932538

VL - 6

SP - 97

EP - 99

JO - ELECTROPHORESIS

JF - ELECTROPHORESIS

SN - 0173-0835

IS - 2

ER -