Abstract
An ultrathin‐layer polyacrylamide gel isoelectric focusing method is described for subtyping transferrin. After a one‐hour pretreatment at room temperature of transferrin in serum with ferrous ammonium sulfate, the samples were subjected to isoelectric focusing for 140 min with a final voltage of 3500 V. Thus, transferrin subtyping was achieved in one‐half of a working day. Population data on transferrin in Black and White samples from Baltimore, Maryland, were obtained using this method. The data were similar to that previously reported on these races from other geographical locations.
Original language | English |
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Pages (from-to) | 97-99 |
Number of pages | 3 |
Journal | Electrophoresis |
Volume | 6 |
Issue number | 2 |
DOIs | |
State | Published - 1 Jan 1985 |