Transferrin subtypes determined by ultrathin‐layer polyacrylamide gel isoelectric focusing

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Abstract

An ultrathin‐layer polyacrylamide gel isoelectric focusing method is described for subtyping transferrin. After a one‐hour pretreatment at room temperature of transferrin in serum with ferrous ammonium sulfate, the samples were subjected to isoelectric focusing for 140 min with a final voltage of 3500 V. Thus, transferrin subtyping was achieved in one‐half of a working day. Population data on transferrin in Black and White samples from Baltimore, Maryland, were obtained using this method. The data were similar to that previously reported on these races from other geographical locations.

Original languageEnglish
Pages (from-to)97-99
Number of pages3
JournalElectrophoresis
Volume6
Issue number2
DOIs
StatePublished - 1 Jan 1985

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