TOS9 regulates white-opaque switching in Candida albicans

Srikantha Thyagarajan, Anthony R. Borneman, Karla J. Daniels, Claude Pujol, Wei Wu, Michael R. Seringhaus, Mark Gerstein, Song Yi, Michael Snyder, David R. Soll

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135 Citations (Scopus)

Abstract

In Candida albicans, the a1-α2 complex represses white-opaque switching, as well as mating. Based upon the assumption that the a1-α2 corepressor complex binds to the gene that regulates white-opaque switching, a chromatin immunoprecipitation-microarray analysis strategy was used to identify 52 genes that bound to the complex. One of these genes, TOS9, exhibited an expression pattern consistent with a "master switch gene." TOS9 was only expressed in opaque cells, and its gene product, Tos9p, localized to the nucleus. Deletion of the gene blocked cells in the white phase, misexpression in the white phase caused stable mass conversion of cells to the opaque state, and misexpression blocked temperature-induced mass conversion from the opaque state to the white state. A model was developed for the regulation of spontaneous switching between the opaque state and the white state that includes stochastic changes of Tos9p levels above and below a threshold that induce changes in the chromatin state of an as-yet-unidentified switching locus. TOS9 has also been referred to as EAP2 and WOR1.

Original languageEnglish
JournalEukaryotic Cell
Volume5
Issue number10
DOIs
StatePublished - 1 Oct 2006

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Candida albicans
Genes
Switch Genes
Co-Repressor Proteins
Chromatin Immunoprecipitation
Gene Deletion
Microarray Analysis
Chromatin
Temperature

Cite this

Thyagarajan, S., Borneman, A. R., Daniels, K. J., Pujol, C., Wu, W., Seringhaus, M. R., ... Soll, D. R. (2006). TOS9 regulates white-opaque switching in Candida albicans. Eukaryotic Cell, 5(10). https://doi.org/10.1128/EC.00252-06
Thyagarajan, Srikantha ; Borneman, Anthony R. ; Daniels, Karla J. ; Pujol, Claude ; Wu, Wei ; Seringhaus, Michael R. ; Gerstein, Mark ; Yi, Song ; Snyder, Michael ; Soll, David R. / TOS9 regulates white-opaque switching in Candida albicans. In: Eukaryotic Cell. 2006 ; Vol. 5, No. 10.
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Thyagarajan, S, Borneman, AR, Daniels, KJ, Pujol, C, Wu, W, Seringhaus, MR, Gerstein, M, Yi, S, Snyder, M & Soll, DR 2006, 'TOS9 regulates white-opaque switching in Candida albicans', Eukaryotic Cell, vol. 5, no. 10. https://doi.org/10.1128/EC.00252-06

TOS9 regulates white-opaque switching in Candida albicans. / Thyagarajan, Srikantha; Borneman, Anthony R.; Daniels, Karla J.; Pujol, Claude; Wu, Wei; Seringhaus, Michael R.; Gerstein, Mark; Yi, Song; Snyder, Michael; Soll, David R.

In: Eukaryotic Cell, Vol. 5, No. 10, 01.10.2006.

Research output: Contribution to journalArticleResearchpeer-review

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Thyagarajan S, Borneman AR, Daniels KJ, Pujol C, Wu W, Seringhaus MR et al. TOS9 regulates white-opaque switching in Candida albicans. Eukaryotic Cell. 2006 Oct 1;5(10). https://doi.org/10.1128/EC.00252-06