TY - JOUR
T1 - TOS9 regulates white-opaque switching in Candida albicans
AU - Srikantha, Thyagarajan
AU - Borneman, Anthony R.
AU - Daniels, Karla J.
AU - Pujol, Claude
AU - Wu, Wei
AU - Seringhaus, Michael R.
AU - Gerstein, Mark
AU - Yi, Song
AU - Snyder, Michael
AU - Soll, David R.
PY - 2006/10
Y1 - 2006/10
N2 - In Candida albicans, the a1-α2 complex represses white-opaque switching, as well as mating. Based upon the assumption that the a1-α2 corepressor complex binds to the gene that regulates white-opaque switching, a chromatin immunoprecipitation-microarray analysis strategy was used to identify 52 genes that bound to the complex. One of these genes, TOS9, exhibited an expression pattern consistent with a "master switch gene." TOS9 was only expressed in opaque cells, and its gene product, Tos9p, localized to the nucleus. Deletion of the gene blocked cells in the white phase, misexpression in the white phase caused stable mass conversion of cells to the opaque state, and misexpression blocked temperature-induced mass conversion from the opaque state to the white state. A model was developed for the regulation of spontaneous switching between the opaque state and the white state that includes stochastic changes of Tos9p levels above and below a threshold that induce changes in the chromatin state of an as-yet-unidentified switching locus. TOS9 has also been referred to as EAP2 and WOR1.
AB - In Candida albicans, the a1-α2 complex represses white-opaque switching, as well as mating. Based upon the assumption that the a1-α2 corepressor complex binds to the gene that regulates white-opaque switching, a chromatin immunoprecipitation-microarray analysis strategy was used to identify 52 genes that bound to the complex. One of these genes, TOS9, exhibited an expression pattern consistent with a "master switch gene." TOS9 was only expressed in opaque cells, and its gene product, Tos9p, localized to the nucleus. Deletion of the gene blocked cells in the white phase, misexpression in the white phase caused stable mass conversion of cells to the opaque state, and misexpression blocked temperature-induced mass conversion from the opaque state to the white state. A model was developed for the regulation of spontaneous switching between the opaque state and the white state that includes stochastic changes of Tos9p levels above and below a threshold that induce changes in the chromatin state of an as-yet-unidentified switching locus. TOS9 has also been referred to as EAP2 and WOR1.
UR - http://www.scopus.com/inward/record.url?scp=33750401562&partnerID=8YFLogxK
U2 - 10.1128/EC.00252-06
DO - 10.1128/EC.00252-06
M3 - Article
C2 - 16950924
AN - SCOPUS:33750401562
SN - 1535-9778
VL - 5
SP - 1674-1687MP
JO - Eukaryotic Cell
JF - Eukaryotic Cell
IS - 10
ER -