TY - JOUR
T1 - Toll-like receptor 2 (TLR2) plays a major role in innate resistance in the lung against murine mycoplasma
AU - Love, Wees
AU - Dobbs, Nicole
AU - Tabor, Leslie
AU - Simecka, Jerry W.
PY - 2010
Y1 - 2010
N2 - Mycoplasma lipoproteins are recognized by Toll-like receptors (TLR), but TLRs' role in responses to infection are unknown. Mycoplasma pulmonis is a naturally occurring respiratory pathogen in mice. In the current study, we used TLR-transfected HEK cells and TLR2-/- bone marrow-derived dendritic cells to demonstrate TLR2-mediated events are important in the initial host-mycoplasma interactions promoting cytokine responses. As we found alveolar macrophages expressed TLR1, TLR2 and TLR6 mRNAs, a role for TLR2 in innate immune clearance in lungs was examined. Three days post-infection, TLR2-/- mice had higher M. pulmonis numbers in lungs, but not in nasal passages. However, TLR2-/- mice had higher lung cytokine levels, indicating TLR2-independent mechanisms are also involved in host responses. Thus, TLR2 plays a critical role in the ability of innate immunity to determine M. pulmonis numbers in the lung, and it is likely that early after respiratory infection that TLR2 recognition of M. pulmonis triggers initial cytokine responses of host cells.
AB - Mycoplasma lipoproteins are recognized by Toll-like receptors (TLR), but TLRs' role in responses to infection are unknown. Mycoplasma pulmonis is a naturally occurring respiratory pathogen in mice. In the current study, we used TLR-transfected HEK cells and TLR2-/- bone marrow-derived dendritic cells to demonstrate TLR2-mediated events are important in the initial host-mycoplasma interactions promoting cytokine responses. As we found alveolar macrophages expressed TLR1, TLR2 and TLR6 mRNAs, a role for TLR2 in innate immune clearance in lungs was examined. Three days post-infection, TLR2-/- mice had higher M. pulmonis numbers in lungs, but not in nasal passages. However, TLR2-/- mice had higher lung cytokine levels, indicating TLR2-independent mechanisms are also involved in host responses. Thus, TLR2 plays a critical role in the ability of innate immunity to determine M. pulmonis numbers in the lung, and it is likely that early after respiratory infection that TLR2 recognition of M. pulmonis triggers initial cytokine responses of host cells.
UR - http://www.scopus.com/inward/record.url?scp=77956281883&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0010739
DO - 10.1371/journal.pone.0010739
M3 - Article
C2 - 20505832
AN - SCOPUS:77956281883
SN - 1932-6203
VL - 5
JO - PLoS ONE
JF - PLoS ONE
IS - 5
M1 - e10739
ER -