@article{53fab9f0e7c8419f9a9572263e02939c,
title = "Tissue Plasminogen Activator-Porous Magnetic Microrods for Targeted Thrombolytic Therapy after Ischemic Stroke",
abstract = "Tissue plasminogen activator (tPA) is the only FDA approved thrombolytic drug for acute ischemic stroke but concerns regarding its limitations remain. Here, we developed a new strategy by incorporating tPA into porous magnetic iron oxide (Fe3O4)-microrods (tPA-MRs) for targeted thrombolytic therapy in ischemic stroke induced by distal middle cerebral artery occlusion. We showed that intra-arterial injection of tPA-MRs could target the cerebral blood clot in vivo under the guidance of an external magnet, where tPA was subsequently released at the site of embolism. When applied with an external rotating magnetic field, rotating tPA-MRs significantly improved not only the mass transport of the tPA-clot reaction, but also mechanically disrupted the clot network, which thus increased clot interaction and penetration of tPA. Importantly, intravenously injected MRs could be discharged from the kidney, and the function of liver and kidney were not damaged at different durations after administration of tPA-MRs. Our data suggest that tPA-MRs overcome the limitations of thrombolytic therapy with tPA alone, which may be not only just for the treatment of ischemic stroke but also have majorly impact on other thrombotic diseases.",
keywords = "ischemia, microrods, stroke, thrombolysis, tissue plasminogen activator",
author = "Jiangnan Hu and Shengwei Huang and Lu Zhu and Weijie Huang and Yiping Zhao and Kunlin Jin and Qichuan Zhuge",
note = "Funding Information: This work is supported by National Science Foundation under the contract ECCS-1303134, National Institutes of Health under the contact of R21 NS084148-01A1, the Funds for International Cooperation and Exchange of the National Natural Science Foundation of China (No. 81820108011), National Natural Scence Foundation of China (No. 81771262), Zhejiang Provincial Key Research and Development Program (2017C03027), National Science Foundation of Beijing (No. 7161014) and Sigma Xi Grants-in-Aid of Research Program Fellowship for Jiangnan Hu (G2017100192773410). Funding Information: This work is supported by National Science Foundation under the contract ECCS-1303134, National Institutes of Health under the contact of R21 NS084148-01A1, the Funds for International Cooperation and Exchange of the National Natural Science Foundation of China (No. 81820108011), National Natural Science Foundation of China (No. 81771262), Zhejiang Provincial Key Research and Development Program (2017C03027), National Science Foundation of Beijing (No. 7161014) and Sigma Xi Grants-in-Aid of Research Program Fellowship for Jiangnan Hu (G2017100192773410). Publisher Copyright: Copyright {\textcopyright} 2018 American Chemical Society.",
year = "2018",
month = oct,
day = "3",
doi = "10.1021/acsami.8b09423",
language = "English",
volume = "10",
pages = "32988--32997",
journal = "ACS Applied Materials and Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "39",
}