TY - JOUR
T1 - Three-photon induced fluorescence of 2,5-diphenyloxazole with a femtosecond Ti:sapphire laser
AU - Gryczynski, Ignacy
AU - Malak, Henryk
AU - Lakowicz, Joseph R.
PY - 1995/10/20
Y1 - 1995/10/20
N2 - We report emission spectra and time-resolved intensity and anisotropy decays resulting from three-photon excitation of fluorescence. The emission intensity of 2,5-diphenyloxazole (PPO) was found to depend on the third power of the excitation intensity at 870 nm. This wavelength is too long to excite PPO by a two-photon process. The emission spectrum, intensity decay, and rotational correlation times were found to be identical for one- (290 nm) and three-photon (870 nm) excitation. However, the time-zero anisotropy (r0 = 0.61) observed from the time-resolved anisotropy decay, and observed in frozen solution, was larger than for one- or two-photon excitation. The possibility of three-photon excitation with a Ti:sapphire laser suggests wide ranging applications in biophysics and fluorescence microscopy.
AB - We report emission spectra and time-resolved intensity and anisotropy decays resulting from three-photon excitation of fluorescence. The emission intensity of 2,5-diphenyloxazole (PPO) was found to depend on the third power of the excitation intensity at 870 nm. This wavelength is too long to excite PPO by a two-photon process. The emission spectrum, intensity decay, and rotational correlation times were found to be identical for one- (290 nm) and three-photon (870 nm) excitation. However, the time-zero anisotropy (r0 = 0.61) observed from the time-resolved anisotropy decay, and observed in frozen solution, was larger than for one- or two-photon excitation. The possibility of three-photon excitation with a Ti:sapphire laser suggests wide ranging applications in biophysics and fluorescence microscopy.
UR - http://www.scopus.com/inward/record.url?scp=0000669110&partnerID=8YFLogxK
U2 - 10.1016/0009-2614(95)00958-7
DO - 10.1016/0009-2614(95)00958-7
M3 - Article
AN - SCOPUS:0000669110
VL - 245
SP - 30
EP - 35
JO - Chemical Physics Letters
JF - Chemical Physics Letters
SN - 0009-2614
IS - 1
ER -