Three-photon induced fluorescence of 2,5-diphenyloxazole with a femtosecond Ti:sapphire laser

Ignacy Gryczynski; Henryk Malak; Joseph R. Lakowicz

doi:10.1016/0009-2614(95)00958-7

Three-photon induced fluorescence of 2,5-diphenyloxazole with a femtosecond Ti:sapphire laser

Ignacy Gryczynski, Henryk Malak, Joseph R. Lakowicz

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

We report emission spectra and time-resolved intensity and anisotropy decays resulting from three-photon excitation of fluorescence. The emission intensity of 2,5-diphenyloxazole (PPO) was found to depend on the third power of the excitation intensity at 870 nm. This wavelength is too long to excite PPO by a two-photon process. The emission spectrum, intensity decay, and rotational correlation times were found to be identical for one- (290 nm) and three-photon (870 nm) excitation. However, the time-zero anisotropy (r₀ = 0.61) observed from the time-resolved anisotropy decay, and observed in frozen solution, was larger than for one- or two-photon excitation. The possibility of three-photon excitation with a Ti:sapphire laser suggests wide ranging applications in biophysics and fluorescence microscopy.

Original language	English
Pages (from-to)	30-35
Number of pages	6
Journal	Chemical Physics Letters
Volume	245
Issue number	1
DOIs	https://doi.org/10.1016/0009-2614(95)00958-7
State	Published - 20 Oct 1995

Access to Document

10.1016/0009-2614(95)00958-7

Link to publication in Scopus

Fingerprint Dive into the research topics of 'Three-photon induced fluorescence of 2,5-diphenyloxazole with a femtosecond Ti:sapphire laser'. Together they form a unique fingerprint.

Cite this

@article{d355440df2274e1c97ffc5ed39ee1a9b,

title = "Three-photon induced fluorescence of 2,5-diphenyloxazole with a femtosecond Ti:sapphire laser",

abstract = "We report emission spectra and time-resolved intensity and anisotropy decays resulting from three-photon excitation of fluorescence. The emission intensity of 2,5-diphenyloxazole (PPO) was found to depend on the third power of the excitation intensity at 870 nm. This wavelength is too long to excite PPO by a two-photon process. The emission spectrum, intensity decay, and rotational correlation times were found to be identical for one- (290 nm) and three-photon (870 nm) excitation. However, the time-zero anisotropy (r0 = 0.61) observed from the time-resolved anisotropy decay, and observed in frozen solution, was larger than for one- or two-photon excitation. The possibility of three-photon excitation with a Ti:sapphire laser suggests wide ranging applications in biophysics and fluorescence microscopy.",

author = "Ignacy Gryczynski and Henryk Malak and Lakowicz, {Joseph R.}",

year = "1995",

month = oct,

day = "20",

doi = "10.1016/0009-2614(95)00958-7",

language = "English",

volume = "245",

pages = "30--35",

journal = "Chemical Physics Letters",

issn = "0009-2614",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Three-photon induced fluorescence of 2,5-diphenyloxazole with a femtosecond Ti:sapphire laser

AU - Gryczynski, Ignacy

AU - Malak, Henryk

AU - Lakowicz, Joseph R.

PY - 1995/10/20

Y1 - 1995/10/20

N2 - We report emission spectra and time-resolved intensity and anisotropy decays resulting from three-photon excitation of fluorescence. The emission intensity of 2,5-diphenyloxazole (PPO) was found to depend on the third power of the excitation intensity at 870 nm. This wavelength is too long to excite PPO by a two-photon process. The emission spectrum, intensity decay, and rotational correlation times were found to be identical for one- (290 nm) and three-photon (870 nm) excitation. However, the time-zero anisotropy (r0 = 0.61) observed from the time-resolved anisotropy decay, and observed in frozen solution, was larger than for one- or two-photon excitation. The possibility of three-photon excitation with a Ti:sapphire laser suggests wide ranging applications in biophysics and fluorescence microscopy.

AB - We report emission spectra and time-resolved intensity and anisotropy decays resulting from three-photon excitation of fluorescence. The emission intensity of 2,5-diphenyloxazole (PPO) was found to depend on the third power of the excitation intensity at 870 nm. This wavelength is too long to excite PPO by a two-photon process. The emission spectrum, intensity decay, and rotational correlation times were found to be identical for one- (290 nm) and three-photon (870 nm) excitation. However, the time-zero anisotropy (r0 = 0.61) observed from the time-resolved anisotropy decay, and observed in frozen solution, was larger than for one- or two-photon excitation. The possibility of three-photon excitation with a Ti:sapphire laser suggests wide ranging applications in biophysics and fluorescence microscopy.

UR - http://www.scopus.com/inward/record.url?scp=0000669110&partnerID=8YFLogxK

U2 - 10.1016/0009-2614(95)00958-7

DO - 10.1016/0009-2614(95)00958-7

M3 - Article

AN - SCOPUS:0000669110

VL - 245

SP - 30

EP - 35

JO - Chemical Physics Letters

JF - Chemical Physics Letters

SN - 0009-2614

IS - 1

ER -