Three-photon induced fluorescence of 2,5-diphenyloxazole with a femtosecond Ti:sapphire laser

Ignacy Gryczynski; Henryk Malak; Joseph R. Lakowicz

doi:10.1016/0009-2614(95)00958-7

Three-photon induced fluorescence of 2,5-diphenyloxazole with a femtosecond Ti:sapphire laser

Ignacy Gryczynski, Henryk Malak, Joseph R. Lakowicz

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Abstract

We report emission spectra and time-resolved intensity and anisotropy decays resulting from three-photon excitation of fluorescence. The emission intensity of 2,5-diphenyloxazole (PPO) was found to depend on the third power of the excitation intensity at 870 nm. This wavelength is too long to excite PPO by a two-photon process. The emission spectrum, intensity decay, and rotational correlation times were found to be identical for one- (290 nm) and three-photon (870 nm) excitation. However, the time-zero anisotropy (r₀ = 0.61) observed from the time-resolved anisotropy decay, and observed in frozen solution, was larger than for one- or two-photon excitation. The possibility of three-photon excitation with a Ti:sapphire laser suggests wide ranging applications in biophysics and fluorescence microscopy.

Original language	English
Pages (from-to)	30-35
Number of pages	6
Journal	Chemical Physics Letters
Volume	245
Issue number	1
DOIs	https://doi.org/10.1016/0009-2614(95)00958-7
State	Published - 20 Oct 1995

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Gryczynski, I., Malak, H., & Lakowicz, J. R. (1995). Three-photon induced fluorescence of 2,5-diphenyloxazole with a femtosecond Ti:sapphire laser. Chemical Physics Letters, 245(1), 30-35. https://doi.org/10.1016/0009-2614(95)00958-7

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abstract = "We report emission spectra and time-resolved intensity and anisotropy decays resulting from three-photon excitation of fluorescence. The emission intensity of 2,5-diphenyloxazole (PPO) was found to depend on the third power of the excitation intensity at 870 nm. This wavelength is too long to excite PPO by a two-photon process. The emission spectrum, intensity decay, and rotational correlation times were found to be identical for one- (290 nm) and three-photon (870 nm) excitation. However, the time-zero anisotropy (r0 = 0.61) observed from the time-resolved anisotropy decay, and observed in frozen solution, was larger than for one- or two-photon excitation. The possibility of three-photon excitation with a Ti:sapphire laser suggests wide ranging applications in biophysics and fluorescence microscopy.",

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AB - We report emission spectra and time-resolved intensity and anisotropy decays resulting from three-photon excitation of fluorescence. The emission intensity of 2,5-diphenyloxazole (PPO) was found to depend on the third power of the excitation intensity at 870 nm. This wavelength is too long to excite PPO by a two-photon process. The emission spectrum, intensity decay, and rotational correlation times were found to be identical for one- (290 nm) and three-photon (870 nm) excitation. However, the time-zero anisotropy (r0 = 0.61) observed from the time-resolved anisotropy decay, and observed in frozen solution, was larger than for one- or two-photon excitation. The possibility of three-photon excitation with a Ti:sapphire laser suggests wide ranging applications in biophysics and fluorescence microscopy.

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