Three-photon excitation of N-acetyl-L-tyrosinamide

Ignacy Gryczynski; Henryk Malak; Joseph R. Lakowicz

doi:10.1016/S0301-4622(99)00033-2

Three-photon excitation of N-acetyl-L-tyrosinamide

Ignacy Gryczynski, Henryk Malak, Joseph R. Lakowicz

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Abstract

We observed emission from the tyrosine derivative N-acetyl-l-tyrosinamide (NATyrA) when excited with the fundamental output of a femtosecond Ti:Sapphire laser from 780 to 855 nm. The dependence on incident laser power indicates a three-photon process. The emission spectra and intensity decay in glycerol-water (30:70) at 5°C were found to be identical for one- and three-photon excitation. Also the excitation spectrum of three-photon-induced fluorescence of NATyrA corresponds to the one-photon excitation spectrum. The time-zero or fundamental anisotropy spectrum was reconstructed from the frequency-domain anisotropy decays. The three-photon anisotropies are similar or larger than the one-photon anisotropies. These three-photon anisotropies are surprising given the near zero values known for tyrosine with two-photon excitation. The observations indicate that one- and three-photon excitation directly populates the same singlet excited states(s). However, the origin of the anisotropies with multi-photon excitation of tyrosine remain unclear and unpredictable. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	25-32
Number of pages	8
Journal	Biophysical Chemistry
Volume	79
Issue number	1
DOIs	https://doi.org/10.1016/S0301-4622(99)00033-2
State	Published - 10 May 1999

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title = "Three-photon excitation of N-acetyl-L-tyrosinamide",

abstract = "We observed emission from the tyrosine derivative N-acetyl-l-tyrosinamide (NATyrA) when excited with the fundamental output of a femtosecond Ti:Sapphire laser from 780 to 855 nm. The dependence on incident laser power indicates a three-photon process. The emission spectra and intensity decay in glycerol-water (30:70) at 5°C were found to be identical for one- and three-photon excitation. Also the excitation spectrum of three-photon-induced fluorescence of NATyrA corresponds to the one-photon excitation spectrum. The time-zero or fundamental anisotropy spectrum was reconstructed from the frequency-domain anisotropy decays. The three-photon anisotropies are similar or larger than the one-photon anisotropies. These three-photon anisotropies are surprising given the near zero values known for tyrosine with two-photon excitation. The observations indicate that one- and three-photon excitation directly populates the same singlet excited states(s). However, the origin of the anisotropies with multi-photon excitation of tyrosine remain unclear and unpredictable. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.",

author = "Ignacy Gryczynski and Henryk Malak and Lakowicz, {Joseph R.}",

note = "Funding Information: This work was supported by a grant from the National Center for Research Resources, RR-08119. ",

year = "1999",

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doi = "10.1016/S0301-4622(99)00033-2",

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pages = "25--32",

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T1 - Three-photon excitation of N-acetyl-L-tyrosinamide

AU - Gryczynski, Ignacy

AU - Malak, Henryk

AU - Lakowicz, Joseph R.

N1 - Funding Information: This work was supported by a grant from the National Center for Research Resources, RR-08119.

PY - 1999/5/10

Y1 - 1999/5/10

N2 - We observed emission from the tyrosine derivative N-acetyl-l-tyrosinamide (NATyrA) when excited with the fundamental output of a femtosecond Ti:Sapphire laser from 780 to 855 nm. The dependence on incident laser power indicates a three-photon process. The emission spectra and intensity decay in glycerol-water (30:70) at 5°C were found to be identical for one- and three-photon excitation. Also the excitation spectrum of three-photon-induced fluorescence of NATyrA corresponds to the one-photon excitation spectrum. The time-zero or fundamental anisotropy spectrum was reconstructed from the frequency-domain anisotropy decays. The three-photon anisotropies are similar or larger than the one-photon anisotropies. These three-photon anisotropies are surprising given the near zero values known for tyrosine with two-photon excitation. The observations indicate that one- and three-photon excitation directly populates the same singlet excited states(s). However, the origin of the anisotropies with multi-photon excitation of tyrosine remain unclear and unpredictable. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.

AB - We observed emission from the tyrosine derivative N-acetyl-l-tyrosinamide (NATyrA) when excited with the fundamental output of a femtosecond Ti:Sapphire laser from 780 to 855 nm. The dependence on incident laser power indicates a three-photon process. The emission spectra and intensity decay in glycerol-water (30:70) at 5°C were found to be identical for one- and three-photon excitation. Also the excitation spectrum of three-photon-induced fluorescence of NATyrA corresponds to the one-photon excitation spectrum. The time-zero or fundamental anisotropy spectrum was reconstructed from the frequency-domain anisotropy decays. The three-photon anisotropies are similar or larger than the one-photon anisotropies. These three-photon anisotropies are surprising given the near zero values known for tyrosine with two-photon excitation. The observations indicate that one- and three-photon excitation directly populates the same singlet excited states(s). However, the origin of the anisotropies with multi-photon excitation of tyrosine remain unclear and unpredictable. Copyright (C) 1999 Elsevier Science B.V. All rights reserved.

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U2 - 10.1016/S0301-4622(99)00033-2

DO - 10.1016/S0301-4622(99)00033-2

M3 - Article

C2 - 10371019

AN - SCOPUS:0032960502

SN - 0301-4622

VL - 79

SP - 25

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JO - Biophysical Chemistry

JF - Biophysical Chemistry

IS - 1

ER -

Three-photon excitation of N-acetyl-L-tyrosinamide

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