Abstract
We show experiments proving the feasibility of scanning fluorescence microscopy by three-photon excitation. Three-photon excitation fluorescence axial images are shown of polystyrene beads stained with the fluorophore 2,5-bis(4-biphenyl)oxazole (BBO). Three-photon excitation is performed at an excitation wavelength of 900 nm and with pulses of 130 fs duration provided by a mode-locked titanium-sapphire laser. Fluorescence is collected between 350 and 450 nm. The fluorescence image signal features a third-order dependence on the excitation power, also providing intrinsic 3-D imaging. The resolution of a three-photon excitation microscope is increased over that of a comparable two-photon excitation microscope.
Original language | English |
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Pages (from-to) | 71-74 |
Number of pages | 4 |
Journal | Journal of Biomedical Optics |
Volume | 1 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1996 |
Keywords
- Fluorescence microscopy
- Resolution
- Three-photon excitation