Thermodynamic Surface Analyses to Inform Biofilm Resistance

T. Brian Cavitt, Jasmine G. Carlisle, Alexandra R. Dodds, Rebecca A. Faulkner, Tyson C. Garfield, Verena N. Ghebranious, Phillip R. Hendley, Emily B. Henry, Charles J. Holt, Jordan R. Lowe, Jacob A. Lowry, D. Spencer Oskin, Pooja R. Patel, Devin Smith, Wenting Wei

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Biofilms are the habitat of 95% of bacteria successfully protecting bacteria from many antibiotics. However, inhibiting biofilm formation is difficult in that it is a complex system involving the physical and chemical interaction of both substrate and bacteria. Focusing on the substrate surface and potential interactions with bacteria, we examined both physical and chemical properties of substrates coated with a series of phenyl acrylate monomer derivatives. Atomic force microscopy (AFM) showed smooth surfaces often approximating surgical grade steel. Induced biofilm growth of five separate bacteria on copolymer samples comprising varying concentrations of phenyl acrylate monomer derivatives evidenced differing degrees of biofilm resistance via optical microscopy. Using goniometric surface analyses, the van Oss-Chaudhury-Good equation was solved linear algebraically to determine the surface energy profile of each polymerized phenyl acrylate monomer derivative, two bacteria, and collagen. Based on the microscopy and surface energy profiles, a thermodynamic explanation for biofilm resistance is posited.

Original languageEnglish
Article number101702
JournaliScience
Volume23
Issue number11
DOIs
StatePublished - 20 Nov 2020

Keywords

  • Microbiology
  • Polymer Chemistry
  • Thermodynamics

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