TY - JOUR
T1 - The WH11 gene of Candida albicans is regulated in two distinct developmental programs through the same transcription activation sequences
AU - Srikantha, Thyagarajan
AU - Tsai, Luong K.
AU - Soll, David R.
PY - 1997/6
Y1 - 1997/6
N2 - Candida albicans strain WO-1 undergoes two developmental programs, the bud-hypha transition and high-frequency phenotypic switching in the form of the white-opaque transition. The WH11 gene is expressed in the white budding phase but is inactive in the white hyphal phase and in the opaque budding phase. WH11 expression, therefore, is regulated in the two developmental programs. Through fusions between deletion derivatives of the WH11 promoter and the newly developed Renilla reniformis luciferase, the WH11 promoter has been characterized in the two developmental programs. Three transcription activation sequences, two strong and one weak, are necessary for the full expression of WH11 in the white budding phase, but no negative regulatory sequences were revealed as playing a role in either the white hyphal phase or the opaque budding phase. These results suggest that regulation is solely through activation in the white budding phase and the same mechanism, therefore, is involved in regulating the differential expression of WH11 in the alternative white and opaque phases of switching and the budding and hyphal phases of dimorphism.
AB - Candida albicans strain WO-1 undergoes two developmental programs, the bud-hypha transition and high-frequency phenotypic switching in the form of the white-opaque transition. The WH11 gene is expressed in the white budding phase but is inactive in the white hyphal phase and in the opaque budding phase. WH11 expression, therefore, is regulated in the two developmental programs. Through fusions between deletion derivatives of the WH11 promoter and the newly developed Renilla reniformis luciferase, the WH11 promoter has been characterized in the two developmental programs. Three transcription activation sequences, two strong and one weak, are necessary for the full expression of WH11 in the white budding phase, but no negative regulatory sequences were revealed as playing a role in either the white hyphal phase or the opaque budding phase. These results suggest that regulation is solely through activation in the white budding phase and the same mechanism, therefore, is involved in regulating the differential expression of WH11 in the alternative white and opaque phases of switching and the budding and hyphal phases of dimorphism.
UR - http://www.scopus.com/inward/record.url?scp=0030995498&partnerID=8YFLogxK
U2 - 10.1128/jb.179.12.3837-3844.1997
DO - 10.1128/jb.179.12.3837-3844.1997
M3 - Article
C2 - 9190797
AN - SCOPUS:0030995498
SN - 0021-9193
VL - 179
SP - 3837
EP - 3844
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 12
ER -