The two-component hybrid kinase regulator CaNIK1 of Candida albicans

Srikantha Thyagarajan, Luong Tsai, Karla Daniels, Lee Enger, Kumiko Highley, David R. Soll

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Abstract

Using degenerate primers of highly conserved regions of two-component response regulators for PCR amplification, a two-component response regulator was cloned from Candida albicans that is homologous to nik-1+ of Neurospora crassa. This two-component hybrid kinase, CaNIK1, also shows features of bacterial two-component response regulators, including a putative unorthodox second histidine kinase motif at the carboxy-terminal end. CaNIK1 was expressed at low levels in both the white and opaque switch phenotypes and in the bud and hyphal growth forms of C. albicans strain WO-1, but in both developmental programmes, the level of transcript was modulated (levels were higher in opaque cells and in hyphae). Partial deletion of both CaNIK1 alleles, by which the histidine autokinase- and ATP-binding domains were removed, did not inhibit either high-frequency phenotypic switching or the bud-hypha transition in high salt concentrations, but in both cases the efficiency of the developmental process was reduced.

Original languageEnglish
Pages (from-to)2715-2729
Number of pages15
JournalMicrobiology
Volume144
Issue number10
DOIs
StatePublished - 1 Jan 1998

Fingerprint

Hyphae
Candida albicans
Phosphotransferases
Neurospora crassa
Histidine
Salts
Adenosine Triphosphate
Alleles
Phenotype
Polymerase Chain Reaction
Growth
Histidine Kinase
autophosphorylation-dependent multifunctional protein kinase

Keywords

  • CaNIK1
  • Candida albicans
  • Histidine kinase
  • Phenotypic switching
  • Two-component hybrid kinase regulator

Cite this

Thyagarajan, S., Tsai, L., Daniels, K., Enger, L., Highley, K., & Soll, D. R. (1998). The two-component hybrid kinase regulator CaNIK1 of Candida albicans. Microbiology, 144(10), 2715-2729. https://doi.org/10.1099/00221287-144-10-2715
Thyagarajan, Srikantha ; Tsai, Luong ; Daniels, Karla ; Enger, Lee ; Highley, Kumiko ; Soll, David R. / The two-component hybrid kinase regulator CaNIK1 of Candida albicans. In: Microbiology. 1998 ; Vol. 144, No. 10. pp. 2715-2729.
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Thyagarajan, S, Tsai, L, Daniels, K, Enger, L, Highley, K & Soll, DR 1998, 'The two-component hybrid kinase regulator CaNIK1 of Candida albicans', Microbiology, vol. 144, no. 10, pp. 2715-2729. https://doi.org/10.1099/00221287-144-10-2715

The two-component hybrid kinase regulator CaNIK1 of Candida albicans. / Thyagarajan, Srikantha; Tsai, Luong; Daniels, Karla; Enger, Lee; Highley, Kumiko; Soll, David R.

In: Microbiology, Vol. 144, No. 10, 01.01.1998, p. 2715-2729.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - The two-component hybrid kinase regulator CaNIK1 of Candida albicans

AU - Thyagarajan, Srikantha

AU - Tsai, Luong

AU - Daniels, Karla

AU - Enger, Lee

AU - Highley, Kumiko

AU - Soll, David R.

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