The two-component hybrid kinase regulator CaNIK1 of Candida albicans

Thyagarajan Srikantha, Luong Tsai, Karla Daniels, Lee Enger, Kumiko Highley, David R. Soll

Research output: Contribution to journalArticlepeer-review

86 Scopus citations


Using degenerate primers of highly conserved regions of two-component response regulators for PCR amplification, a two-component response regulator was cloned from Candida albicans that is homologous to nik-1+ of Neurospora crassa. This two-component hybrid kinase, CaNIK1, also shows features of bacterial two-component response regulators, including a putative unorthodox second histidine kinase motif at the carboxy-terminal end. CaNIK1 was expressed at low levels in both the white and opaque switch phenotypes and in the bud and hyphal growth forms of C. albicans strain WO-1, but in both developmental programmes, the level of transcript was modulated (levels were higher in opaque cells and in hyphae). Partial deletion of both CaNIK1 alleles, by which the histidine autokinase- and ATP-binding domains were removed, did not inhibit either high-frequency phenotypic switching or the bud-hypha transition in high salt concentrations, but in both cases the efficiency of the developmental process was reduced.

Original languageEnglish
Pages (from-to)2715-2729
Number of pages15
Issue number10
StatePublished - Oct 1998


  • CaNIK1
  • Candida albicans
  • Histidine kinase
  • Phenotypic switching
  • Two-component hybrid kinase regulator


Dive into the research topics of 'The two-component hybrid kinase regulator CaNIK1 of Candida albicans'. Together they form a unique fingerprint.

Cite this