The telomerase reverse transcriptase is limiting and necessary for telomerase function in vivo

Y. Liu; B. E. Snow; M. P. Hande; D. Yeung; N. J. Erdmann; A. Wakeham; A. Itie; D. P. Siderovski; P. M. Lansdorp; M. O. Robinson; L. Harrington

doi:10.1016/S0960-9822(00)00805-8

The telomerase reverse transcriptase is limiting and necessary for telomerase function in vivo

Y. Liu, B. E. Snow, M. P. Hande, D. Yeung, N. J. Erdmann, A. Wakeham, A. Itie, D. P. Siderovski, P. M. Lansdorp, M. O. Robinson, L. Harrington

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Abstract

Mammalian telomerase is essential for the maintenance of telomere length [1-5]. Its catalytic core comprises a reverse transcriptase component (TERT) and an RNA component. While the biochemical role of mammalian TERT is well established [6-11], it is unknown whether it is sufficient for telomere-length maintenance, chromosome stability or other cellular processes. Cells from mice in which the mTert gene had been disrupted showed progressive loss of telomere DNA, a phenotype similar to cells in which the gene encoding the telomerase RNA component (mTR) has been disrupted [1,12]. On prolonged growth, mTert-deficient embryonic stem (ES) cells exhibited genomic instability, aneuploidy and telomeric fusions. ES cells heterozygous for the mTert disruption also showed telomere attrition, a phenotype that differs from heterozygous mTR cells [12]. Thus, telomere maintenance in mammals is carried out by a single, limiting TERT.

Original language	English
Pages (from-to)	1459-1462
Number of pages	4
Journal	Current Biology
Volume	10
Issue number	22
DOIs	https://doi.org/10.1016/S0960-9822(00)00805-8
State	Published - 16 Nov 2000

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title = "The telomerase reverse transcriptase is limiting and necessary for telomerase function in vivo",

abstract = "Mammalian telomerase is essential for the maintenance of telomere length [1-5]. Its catalytic core comprises a reverse transcriptase component (TERT) and an RNA component. While the biochemical role of mammalian TERT is well established [6-11], it is unknown whether it is sufficient for telomere-length maintenance, chromosome stability or other cellular processes. Cells from mice in which the mTert gene had been disrupted showed progressive loss of telomere DNA, a phenotype similar to cells in which the gene encoding the telomerase RNA component (mTR) has been disrupted [1,12]. On prolonged growth, mTert-deficient embryonic stem (ES) cells exhibited genomic instability, aneuploidy and telomeric fusions. ES cells heterozygous for the mTert disruption also showed telomere attrition, a phenotype that differs from heterozygous mTR cells [12]. Thus, telomere maintenance in mammals is carried out by a single, limiting TERT.",

author = "Y. Liu and Snow, {B. E.} and Hande, {M. P.} and D. Yeung and Erdmann, {N. J.} and A. Wakeham and A. Itie and Siderovski, {D. P.} and Lansdorp, {P. M.} and Robinson, {M. O.} and L. Harrington",

note = "Funding Information: We thank C. Greider, S. Le, M. Hemann, T. Mak, and the laboratory for discussion, and D. Bouchard and M. Ungrin for assistance with Flow–FISH. Y.L. is a research fellow of the National Cancer Institute of Canada supported with funds provided by the Terry Fox Run. L.H. acknowledges the support of the Medical Research Council and the National Institute of Health (AG8422117). ",

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doi = "10.1016/S0960-9822(00)00805-8",

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T1 - The telomerase reverse transcriptase is limiting and necessary for telomerase function in vivo

AU - Liu, Y.

AU - Snow, B. E.

AU - Hande, M. P.

AU - Yeung, D.

AU - Erdmann, N. J.

AU - Wakeham, A.

AU - Itie, A.

AU - Siderovski, D. P.

AU - Lansdorp, P. M.

AU - Robinson, M. O.

AU - Harrington, L.

N1 - Funding Information: We thank C. Greider, S. Le, M. Hemann, T. Mak, and the laboratory for discussion, and D. Bouchard and M. Ungrin for assistance with Flow–FISH. Y.L. is a research fellow of the National Cancer Institute of Canada supported with funds provided by the Terry Fox Run. L.H. acknowledges the support of the Medical Research Council and the National Institute of Health (AG8422117).

PY - 2000/11/16

Y1 - 2000/11/16

N2 - Mammalian telomerase is essential for the maintenance of telomere length [1-5]. Its catalytic core comprises a reverse transcriptase component (TERT) and an RNA component. While the biochemical role of mammalian TERT is well established [6-11], it is unknown whether it is sufficient for telomere-length maintenance, chromosome stability or other cellular processes. Cells from mice in which the mTert gene had been disrupted showed progressive loss of telomere DNA, a phenotype similar to cells in which the gene encoding the telomerase RNA component (mTR) has been disrupted [1,12]. On prolonged growth, mTert-deficient embryonic stem (ES) cells exhibited genomic instability, aneuploidy and telomeric fusions. ES cells heterozygous for the mTert disruption also showed telomere attrition, a phenotype that differs from heterozygous mTR cells [12]. Thus, telomere maintenance in mammals is carried out by a single, limiting TERT.

AB - Mammalian telomerase is essential for the maintenance of telomere length [1-5]. Its catalytic core comprises a reverse transcriptase component (TERT) and an RNA component. While the biochemical role of mammalian TERT is well established [6-11], it is unknown whether it is sufficient for telomere-length maintenance, chromosome stability or other cellular processes. Cells from mice in which the mTert gene had been disrupted showed progressive loss of telomere DNA, a phenotype similar to cells in which the gene encoding the telomerase RNA component (mTR) has been disrupted [1,12]. On prolonged growth, mTert-deficient embryonic stem (ES) cells exhibited genomic instability, aneuploidy and telomeric fusions. ES cells heterozygous for the mTert disruption also showed telomere attrition, a phenotype that differs from heterozygous mTR cells [12]. Thus, telomere maintenance in mammals is carried out by a single, limiting TERT.

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The telomerase reverse transcriptase is limiting and necessary for telomerase function in vivo

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