The similarity of protein expression in trabecular meshwork and lamina cribrosa: Implications for glaucoma

H. Thomas Steely, Sherry L. English-Wright, Abbot Clark

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The purpose of the present investigation was to compare protein expression in various ocular cells and tissues including the human trabecular meshwork (TM) and the lamina cribrosa (LC). To conduct the comparisons, we primarily utilized autofluorography of one-dimensional (1D) and high resolution, two-dimensional (2D) polyacrylamide gels of proteins from radiolabelled tissues and cultured cells. Results from the investigations indicated that patterns of protein expression from TM and LC were the most similar among the ocular cells and tissues compared. Specifically, these autofluorographic 'fingerprints' indicated that proteins in TM and LC cultured cells and tissue were exceptionally similar (a) in band position and intensity (1D gels) and (b) in spot congruence (2D gels) as compared to other ocular cells and tissues. We conclude that the TM and the LC, two ocular tissues intimately linked to the pathogenesis of primary open-angle glaucoma, display remarkable similarity in protein expression. This finding may have implications for the molecular etiology of glaucoma.

Original languageEnglish
Pages (from-to)17-30
Number of pages14
JournalExperimental eye research
Volume70
Issue number1
DOIs
StatePublished - 1 Jan 2000

Keywords

  • 2D-PAGE Electrophoresis
  • Lamina cribrosa
  • Primary open-angle glaucoma
  • Protein expression
  • Proteomics
  • Trabecular meshwork

Fingerprint Dive into the research topics of 'The similarity of protein expression in trabecular meshwork and lamina cribrosa: Implications for glaucoma'. Together they form a unique fingerprint.

  • Cite this