Abstract
Heterotrimeric G-proteins are molecular switches that convert signals from membrane receptors into changes in intracellular physiology. Recently, several peptides that bind heterotrimeric G-protein α subunits have been isolated including the novel Gαi1 • GDP binding peptides R6A and KB-752. The R6A peptide and its minimized derivative R6A-1 interact with Gαi1 • GDP. Based on spectroscopic analysis of BODIPYFL-GTPγS binding to Gαi1, it has been reported that R6A-1 has guanine nucleotide dissociation inhibitor (GDI) activity against Gαi1 [W.W. Ja, R.W. Roberts, Biochemistry 43 (28) (2004) 9265-9275]. Using radioligand binding, we show that R6A-1 is not a GDI for Gαi1 subunits. Furthermore, we demonstrate that R6A-1 reduces the fluorescence quantum yield of the Gαi1-BODIPYFL-GTPγS complex, thus explaining the previously reported GDI activity as a fluorescence artifact. We further show that R6A-1 has significant sequence similarity to the guanine nucleotide exchange factor peptide KB-752 that binds to switch II of Gαi1. We use competitive binding analysis to show that R6A-1 also binds to switch II of Gα subunits.
Original language | English |
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Pages (from-to) | 1107-1112 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 339 |
Issue number | 4 |
DOIs | |
State | Published - 27 Jan 2006 |
Keywords
- BODIPYFL-GTPγS
- G-protein
- GDI
- GoLoco motif
- Guanine nucleotide dissociation inhibitor
- KB-752
- R6A
- R6A-1
- Switch region