Heterotrimeric G-proteins are molecular switches that convert signals from membrane receptors into changes in intracellular physiology. Recently, several peptides that bind heterotrimeric G-protein α subunits have been isolated including the novel Gαi1 • GDP binding peptides R6A and KB-752. The R6A peptide and its minimized derivative R6A-1 interact with Gαi1 • GDP. Based on spectroscopic analysis of BODIPYFL-GTPγS binding to Gαi1, it has been reported that R6A-1 has guanine nucleotide dissociation inhibitor (GDI) activity against Gαi1 [W.W. Ja, R.W. Roberts, Biochemistry 43 (28) (2004) 9265-9275]. Using radioligand binding, we show that R6A-1 is not a GDI for Gαi1 subunits. Furthermore, we demonstrate that R6A-1 reduces the fluorescence quantum yield of the Gαi1-BODIPYFL-GTPγS complex, thus explaining the previously reported GDI activity as a fluorescence artifact. We further show that R6A-1 has significant sequence similarity to the guanine nucleotide exchange factor peptide KB-752 that binds to switch II of Gαi1. We use competitive binding analysis to show that R6A-1 also binds to switch II of Gα subunits.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - 27 Jan 2006|
- GoLoco motif
- Guanine nucleotide dissociation inhibitor
- Switch region