The protein scaffold of the lipocalin odorant-binding protein is suitable for the design of new biosensors for the detection of explosive components

Roberto Ramoni, Stefano Bellucci, Ignacy Grycznyski, Zygmunt Gryczynski, Stefano Grolli, Maria Staiano, Giovanni De Bellis, Federico Micciulla, Roberto Pastore, Alessandra Tiberia, Virna Conti, Elisa Merli, Antonio Varriale, Mose' Rossi, Sabato D'Auria

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The detection of hazard exposure is a current priority, including the detection of traces of explosive molecules in different environments like luggage storage rooms and public places, and is becoming a major requirement for homeland security. In the present study we carried out a preliminary investigation on the binding capacities of four forms of the lipocalin odorant-binding protein (OBP) for the detection of explosive components such as diphenylamine, dimethyl-phthalate, resorcinol and dinitrotoluene. The experimental results, showing that OBP binds these compounds with affinity constants ranging between 80nM and 10.6mM, indicate that this protein can be used as a probe for the realization of a biosensor to sense explosive compounds.

Original languageEnglish
Article number395012
JournalJournal of Physics Condensed Matter
Volume19
Issue number39
DOIs
StatePublished - 3 Oct 2007

Fingerprint

Lipocalins
Scaffolds (biology)
bioinstrumentation
Biosensors
Scaffolds
proteins
Proteins
Diphenylamine
phthalates
National security
hazards
rooms
affinity
Hazards
requirements
Molecules
probes
odorant-binding protein
Carrier Proteins
molecules

Cite this

Ramoni, Roberto ; Bellucci, Stefano ; Grycznyski, Ignacy ; Gryczynski, Zygmunt ; Grolli, Stefano ; Staiano, Maria ; De Bellis, Giovanni ; Micciulla, Federico ; Pastore, Roberto ; Tiberia, Alessandra ; Conti, Virna ; Merli, Elisa ; Varriale, Antonio ; Rossi, Mose' ; D'Auria, Sabato. / The protein scaffold of the lipocalin odorant-binding protein is suitable for the design of new biosensors for the detection of explosive components. In: Journal of Physics Condensed Matter. 2007 ; Vol. 19, No. 39.
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Ramoni, R, Bellucci, S, Grycznyski, I, Gryczynski, Z, Grolli, S, Staiano, M, De Bellis, G, Micciulla, F, Pastore, R, Tiberia, A, Conti, V, Merli, E, Varriale, A, Rossi, M & D'Auria, S 2007, 'The protein scaffold of the lipocalin odorant-binding protein is suitable for the design of new biosensors for the detection of explosive components', Journal of Physics Condensed Matter, vol. 19, no. 39, 395012. https://doi.org/10.1088/0953-8984/19/39/395012

The protein scaffold of the lipocalin odorant-binding protein is suitable for the design of new biosensors for the detection of explosive components. / Ramoni, Roberto; Bellucci, Stefano; Grycznyski, Ignacy; Gryczynski, Zygmunt; Grolli, Stefano; Staiano, Maria; De Bellis, Giovanni; Micciulla, Federico; Pastore, Roberto; Tiberia, Alessandra; Conti, Virna; Merli, Elisa; Varriale, Antonio; Rossi, Mose'; D'Auria, Sabato.

In: Journal of Physics Condensed Matter, Vol. 19, No. 39, 395012, 03.10.2007.

Research output: Contribution to journalArticle

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AU - Tiberia, Alessandra

AU - Conti, Virna

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AU - Varriale, Antonio

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