The protein scaffold of the lipocalin odorant-binding protein is suitable for the design of new biosensors for the detection of explosive components

Roberto Ramoni, Stefano Bellucci, Ignacy Grycznyski, Zigmunt Grycznyski, Stefano Grolli, Maria Staiano, Giovanni De Bellis, Federico Micciulla, Roberto Pastore, Alessandra Tiberia, Virna Conti, Elisa Merli, Antonio Varriale, Mose' Rossi, Sabato D'Auria

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The detection of hazard exposure is a current priority, including the detection of traces of explosive molecules in different environments like luggage storage rooms and public places, and is becoming a major requirement for homeland security. In the present study we carried out a preliminary investigation on the binding capacities of four forms of the lipocalin odorant-binding protein (OBP) for the detection of explosive components such as diphenylamine, dimethyl-phthalate, resorcinol and dinitrotoluene. The experimental results, showing that OBP binds these compounds with affinity constants ranging between 80nM and 10.6mM, indicate that this protein can be used as a probe for the realization of a biosensor to sense explosive compounds.

Original languageEnglish
Article number395012
JournalJournal of Physics Condensed Matter
Volume19
Issue number39
DOIs
StatePublished - 3 Oct 2007

Fingerprint Dive into the research topics of 'The protein scaffold of the lipocalin odorant-binding protein is suitable for the design of new biosensors for the detection of explosive components'. Together they form a unique fingerprint.

Cite this