Abstract
1. 1. Active Cl transport occurs from the endothelial to epithelial side of the cornea of Bufo marinus. Na transport is much less and is in the opposite direction. 2. 2. The rate of Cl transport is not saturable and is linearly related to the Cl concentration on the endothelial side. 3. 3. Active efflux (endo to epi) of Cl is reduced (50%) by CN and abolished by IAA. Ouabain and Na-free solutions on the endothelial side also reduce Cl efflux. 4. 4. O2 consumption or lactate production are also decreased by ouabain or Na-free solutions. However, metabolism was not inhibited by Cl-free solutions or a specific Cl blocking drug bumetanide. 5. 5. Cl transport exhibits some rather unusual characters and a model is proposed to account for them which involves an exchange of Cl for metabolically produced organic anions.
| Original language | English |
|---|---|
| Pages (from-to) | 635-641 |
| Number of pages | 7 |
| Journal | Comparative Biochemistry and Physiology -- Part A: Physiology |
| Volume | 61 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1 Jan 1978 |
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The properties of "active" Cl transport across the cornea of the toad Bufo marinus. / Bentley, P. J.; Yorio, Thomas.
In: Comparative Biochemistry and Physiology -- Part A: Physiology, Vol. 61, No. 4, 01.01.1978, p. 635-641.Research output: Contribution to journal › Article
TY - JOUR
T1 - The properties of "active" Cl transport across the cornea of the toad Bufo marinus
AU - Bentley, P. J.
AU - Yorio, Thomas
PY - 1978/1/1
Y1 - 1978/1/1
N2 - 1. 1. Active Cl transport occurs from the endothelial to epithelial side of the cornea of Bufo marinus. Na transport is much less and is in the opposite direction. 2. 2. The rate of Cl transport is not saturable and is linearly related to the Cl concentration on the endothelial side. 3. 3. Active efflux (endo to epi) of Cl is reduced (50%) by CN and abolished by IAA. Ouabain and Na-free solutions on the endothelial side also reduce Cl efflux. 4. 4. O2 consumption or lactate production are also decreased by ouabain or Na-free solutions. However, metabolism was not inhibited by Cl-free solutions or a specific Cl blocking drug bumetanide. 5. 5. Cl transport exhibits some rather unusual characters and a model is proposed to account for them which involves an exchange of Cl for metabolically produced organic anions.
AB - 1. 1. Active Cl transport occurs from the endothelial to epithelial side of the cornea of Bufo marinus. Na transport is much less and is in the opposite direction. 2. 2. The rate of Cl transport is not saturable and is linearly related to the Cl concentration on the endothelial side. 3. 3. Active efflux (endo to epi) of Cl is reduced (50%) by CN and abolished by IAA. Ouabain and Na-free solutions on the endothelial side also reduce Cl efflux. 4. 4. O2 consumption or lactate production are also decreased by ouabain or Na-free solutions. However, metabolism was not inhibited by Cl-free solutions or a specific Cl blocking drug bumetanide. 5. 5. Cl transport exhibits some rather unusual characters and a model is proposed to account for them which involves an exchange of Cl for metabolically produced organic anions.
UR - http://www.scopus.com/inward/record.url?scp=0018079047&partnerID=8YFLogxK
U2 - 10.1016/0300-9629(78)90141-X
DO - 10.1016/0300-9629(78)90141-X
M3 - Article
AN - SCOPUS:0018079047
VL - 61
SP - 635
EP - 641
JO - Comparative Biochemistry and Physiology -- Part A: Physiology
JF - Comparative Biochemistry and Physiology -- Part A: Physiology
SN - 0300-9629
IS - 4
ER -