TY - JOUR
T1 - The presence of anti-LPS antibodies and human serum activity against Proteus mirabilis S/R forms in correlation with TLR4 (Thr399Ile) gene polymorphism in rheumatoid arthritis
AU - Arabski, Michal
AU - Fudala, Rafal
AU - Koza, Anna
AU - Wasik, Slawomir
AU - Futoma-Koloch, Bozena
AU - Bugla-Ploskonska, Gabriela
AU - Kaca, Wieslaw
N1 - Funding Information:
The work was supported by grant BS/2012 from the Jan Kochanowski University, Poland . We would like to thank Krystyna Surma, Iwona Koprucha, Halina Bakalarska, Wojciech Garbacz, Łukasz Madej, Ewelina Nowak and Agnieszka Matusiak for sample collection and technical support.
PY - 2012/11
Y1 - 2012/11
N2 - Objectives: Proteus mirabilis strains are human pathogens responsible for urinary tract infections, which may also be involved in rheumatoid arthritis (RA). Design and methods: We determined whether the binding site of anti-LPS antibodies on the O-polysaccharide part of P. mirabilis LPS correlates with the level of TLR4 (Thr399Ile) gene polymorphism in the sera of RA patients. We investigated the deposition of C3d and C5b complement components on the P. mirabilis LPS. The ELISA method used in this study was optimized with LAL test and laser interferometry. Results: Depending on LPS P. mirabilis used in these studies, the amount of antibodies in RA patients sera varied. We did not observe a correlation between anti-LPS antibodies binding and the level of TLR4 (Thr399Ile) gene polymorphism. We found that the lower complement components deposition by O49 in contrast to O9 LPS correlates with its reduced sensitivities to human complement-mediated killing. Conclusion: The immunological response against P. mirabilis LPS might play a role in rheumatoid arthritis.
AB - Objectives: Proteus mirabilis strains are human pathogens responsible for urinary tract infections, which may also be involved in rheumatoid arthritis (RA). Design and methods: We determined whether the binding site of anti-LPS antibodies on the O-polysaccharide part of P. mirabilis LPS correlates with the level of TLR4 (Thr399Ile) gene polymorphism in the sera of RA patients. We investigated the deposition of C3d and C5b complement components on the P. mirabilis LPS. The ELISA method used in this study was optimized with LAL test and laser interferometry. Results: Depending on LPS P. mirabilis used in these studies, the amount of antibodies in RA patients sera varied. We did not observe a correlation between anti-LPS antibodies binding and the level of TLR4 (Thr399Ile) gene polymorphism. We found that the lower complement components deposition by O49 in contrast to O9 LPS correlates with its reduced sensitivities to human complement-mediated killing. Conclusion: The immunological response against P. mirabilis LPS might play a role in rheumatoid arthritis.
KW - Complement
KW - LAL
KW - Laser interferometry
KW - Proteus mirabilis, lipopolysaccharides
KW - Rheumatoid arthritis
KW - TLR4 gene polymorphism
UR - http://www.scopus.com/inward/record.url?scp=84868301270&partnerID=8YFLogxK
U2 - 10.1016/j.clinbiochem.2012.06.021
DO - 10.1016/j.clinbiochem.2012.06.021
M3 - Article
C2 - 22749779
AN - SCOPUS:84868301270
SN - 0009-9120
VL - 45
SP - 1374
EP - 1382
JO - Clinical Biochemistry
JF - Clinical Biochemistry
IS - 16-17
ER -