Abstract
The application of DNA typing methods after amplification by the polymerase chain reaction (PCR) of DNA derived from body tissues from charred fire victims was investigated. A total of 26 different tissue specimens from ten extensively burned individuals were analyzed. The samples included femoral muscle, psoas muscle, bone marrow and blood. The post-mortem period varied from 38 to 183 h. After amplifying the DNA by PCR from the various tissues, the D1S80 locus was analyzed with a high resolution polyacrylamide gel electrophoresis technique followed by silver staining and the alleles of the HLA-DQα locus were detected by using a reverse dot blot format. All samples could be typed for both loci and the genotypes were consistent in the various tissues from each individual. A parentage test was performed in two cases and Mendelian inheritance of the alleles for both loci was observed.
Original language | English |
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Pages (from-to) | 23-34 |
Number of pages | 12 |
Journal | Forensic Science International |
Volume | 51 |
Issue number | 1 |
DOIs | |
State | Published - Oct 1991 |
Keywords
- Fire victims
- Forensic science
- Identity testing
- Polymerase chain reaction
- Post-mortem