The polymerase chain reaction and post-mortem forensic identity testing: Application of amplified D1S80 and HLA-DQα loci to the identification of fire victims

A. Sajantila, M. Ström, B. Budowle, P. J. Karhunen, L. Peltonen

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

The application of DNA typing methods after amplification by the polymerase chain reaction (PCR) of DNA derived from body tissues from charred fire victims was investigated. A total of 26 different tissue specimens from ten extensively burned individuals were analyzed. The samples included femoral muscle, psoas muscle, bone marrow and blood. The post-mortem period varied from 38 to 183 h. After amplifying the DNA by PCR from the various tissues, the D1S80 locus was analyzed with a high resolution polyacrylamide gel electrophoresis technique followed by silver staining and the alleles of the HLA-DQα locus were detected by using a reverse dot blot format. All samples could be typed for both loci and the genotypes were consistent in the various tissues from each individual. A parentage test was performed in two cases and Mendelian inheritance of the alleles for both loci was observed.

Original languageEnglish
Pages (from-to)23-34
Number of pages12
JournalForensic Science International
Volume51
Issue number1
DOIs
StatePublished - Oct 1991

Keywords

  • Fire victims
  • Forensic science
  • Identity testing
  • Polymerase chain reaction
  • Post-mortem

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