The polymerase chain reaction and post-mortem forensic identity testing: Application of amplified D1S80 and HLA-DQα loci to the identification of fire victims

A. Sajantila; M. Ström; B. Budowle; P. J. Karhunen; L. Peltonen

doi:10.1016/0379-0738(91)90203-U

The polymerase chain reaction and post-mortem forensic identity testing: Application of amplified D1S80 and HLA-DQα loci to the identification of fire victims

A. Sajantila, M. Ström, B. Budowle, P. J. Karhunen, L. Peltonen

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17 Scopus citations

Abstract

The application of DNA typing methods after amplification by the polymerase chain reaction (PCR) of DNA derived from body tissues from charred fire victims was investigated. A total of 26 different tissue specimens from ten extensively burned individuals were analyzed. The samples included femoral muscle, psoas muscle, bone marrow and blood. The post-mortem period varied from 38 to 183 h. After amplifying the DNA by PCR from the various tissues, the D1S80 locus was analyzed with a high resolution polyacrylamide gel electrophoresis technique followed by silver staining and the alleles of the HLA-DQα locus were detected by using a reverse dot blot format. All samples could be typed for both loci and the genotypes were consistent in the various tissues from each individual. A parentage test was performed in two cases and Mendelian inheritance of the alleles for both loci was observed.

Original language	English
Pages (from-to)	23-34
Number of pages	12
Journal	Forensic Science International
Volume	51
Issue number	1
DOIs	https://doi.org/10.1016/0379-0738(91)90203-U
State	Published - Oct 1991

Keywords

Fire victims
Forensic science
Identity testing
Polymerase chain reaction
Post-mortem

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10.1016/0379-0738(91)90203-U

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title = "The polymerase chain reaction and post-mortem forensic identity testing: Application of amplified D1S80 and HLA-DQα loci to the identification of fire victims",

abstract = "The application of DNA typing methods after amplification by the polymerase chain reaction (PCR) of DNA derived from body tissues from charred fire victims was investigated. A total of 26 different tissue specimens from ten extensively burned individuals were analyzed. The samples included femoral muscle, psoas muscle, bone marrow and blood. The post-mortem period varied from 38 to 183 h. After amplifying the DNA by PCR from the various tissues, the D1S80 locus was analyzed with a high resolution polyacrylamide gel electrophoresis technique followed by silver staining and the alleles of the HLA-DQα locus were detected by using a reverse dot blot format. All samples could be typed for both loci and the genotypes were consistent in the various tissues from each individual. A parentage test was performed in two cases and Mendelian inheritance of the alleles for both loci was observed.",

keywords = "Fire victims, Forensic science, Identity testing, Polymerase chain reaction, Post-mortem",

author = "A. Sajantila and M. Str{\"o}m and B. Budowle and Karhunen, {P. J.} and L. Peltonen",

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AU - Ström, M.

AU - Budowle, B.

AU - Karhunen, P. J.

AU - Peltonen, L.

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AB - The application of DNA typing methods after amplification by the polymerase chain reaction (PCR) of DNA derived from body tissues from charred fire victims was investigated. A total of 26 different tissue specimens from ten extensively burned individuals were analyzed. The samples included femoral muscle, psoas muscle, bone marrow and blood. The post-mortem period varied from 38 to 183 h. After amplifying the DNA by PCR from the various tissues, the D1S80 locus was analyzed with a high resolution polyacrylamide gel electrophoresis technique followed by silver staining and the alleles of the HLA-DQα locus were detected by using a reverse dot blot format. All samples could be typed for both loci and the genotypes were consistent in the various tissues from each individual. A parentage test was performed in two cases and Mendelian inheritance of the alleles for both loci was observed.

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KW - Identity testing

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KW - Post-mortem

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The polymerase chain reaction and post-mortem forensic identity testing: Application of amplified D1S80 and HLA-DQα loci to the identification of fire victims

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Keywords

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