The NKp46 receptor contributes to NK cell lysis of mononuclear phagocytes infected with an intracellular bacterium

Ramakrishna Vankayalapati, Benjamin Wizel, Stephen E. Weis, Hassan Safi, David L. Lakey, Ofer Mandelboim, Buka Samten, Angel Porgador, Peter F. Barnes

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

We used human tuberculosis as a model to investigate the role of NK cytotoxic mechanisms in the immune response to intracellular infection. Freshly isolated NK cells and NK cell lines from healthy donors lysed Mycobacterium tuberculosis-infected monocytes to a greater extent than uninfected monocytes. Lysis of infected monocytes was associated with increased expression of mRNA for the NKp46 receptor, but not the NKp44 receptor. Antisera to NKp46 markedly inhibited lysis of infected monocytes. NK cell-mediated lysis was not due to reduced expression of MHC class I molecules on the surface of infected monocytes or to enhanced production of IL-18 or IFN-γ. NK cell lytic activity against M. tuberculosis-infected monocytes and NKp46 mRNA expression were reduced in tuberculosis patients with ineffective immunity to M. tuberculosis compared with findings in healthy donors. These observations suggest that 1) the NKp46 receptor participates in NK cell-mediated lysis of cells infected with an intracellular pathogen, and 2) the reduced functional capacity of NK cells is associated with severe manifestations of infectious disease.

Original languageEnglish
Pages (from-to)3451-3457
Number of pages7
JournalJournal of Immunology
Volume168
Issue number7
DOIs
StatePublished - 1 Apr 2002

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Natural Cytotoxicity Triggering Receptor 1
Phagocytes
Natural Killer Cells
Monocytes
Bacteria
Mycobacterium tuberculosis
Natural Cytotoxicity Triggering Receptor 2
Tuberculosis
Tissue Donors
Messenger RNA
Interleukin-18
Communicable Diseases
Immune Sera
Immunity
Cell Line

Cite this

Vankayalapati, Ramakrishna ; Wizel, Benjamin ; Weis, Stephen E. ; Safi, Hassan ; Lakey, David L. ; Mandelboim, Ofer ; Samten, Buka ; Porgador, Angel ; Barnes, Peter F. / The NKp46 receptor contributes to NK cell lysis of mononuclear phagocytes infected with an intracellular bacterium. In: Journal of Immunology. 2002 ; Vol. 168, No. 7. pp. 3451-3457.
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Vankayalapati, R, Wizel, B, Weis, SE, Safi, H, Lakey, DL, Mandelboim, O, Samten, B, Porgador, A & Barnes, PF 2002, 'The NKp46 receptor contributes to NK cell lysis of mononuclear phagocytes infected with an intracellular bacterium', Journal of Immunology, vol. 168, no. 7, pp. 3451-3457. https://doi.org/10.4049/jimmunol.168.7.3451

The NKp46 receptor contributes to NK cell lysis of mononuclear phagocytes infected with an intracellular bacterium. / Vankayalapati, Ramakrishna; Wizel, Benjamin; Weis, Stephen E.; Safi, Hassan; Lakey, David L.; Mandelboim, Ofer; Samten, Buka; Porgador, Angel; Barnes, Peter F.

In: Journal of Immunology, Vol. 168, No. 7, 01.04.2002, p. 3451-3457.

Research output: Contribution to journalArticle

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AU - Wizel, Benjamin

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AU - Samten, Buka

AU - Porgador, Angel

AU - Barnes, Peter F.

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