The new Standard Reference Material® 2391c: PCR-based DNA profiling standard

M. C. Kline, E. L.R. Butts, C. R. Hill, Michael Dewitt Coble, D. L. Duewer, J. M. Butler

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Standard Reference Material® 2391c (SRM 2391c) is the fourth generation certified reference material for PCR-based DNA profiling. The first generation SRM 2391 was released in 1995; the next two subsequent versions have had minor modifications in the type and number of loci certified but always used DNA from the same donors. SRM 2391c has been produced with an entirely new set of genomic DNA samples. In addition to four liquid samples, SRM 2391c has two dry storage matrices including FTA paper as well as 903 paper. SRM 2391c consists of six components: three are single source genomic DNA samples that are labeled A, B, and C, with the fourth genomic sample (component D) as a mixture of 3 parts of components A (female donor) to 1 part of component C (male donor). Component E consists of two 6. mm punches of 903 paper that have been spotted with approximately 75,000 cells/spot. Component F consists of two 6. mm punches of FTA paper that have been spotted with approximately 75,000 cells/spot of a different cell line. The six components representing five different DNA samples plus the mixture component have been analyzed using 22 commercially available STR typing kits, obtained from three different vendors, as well as the 26plex STR multiplex developed at NIST. In total there are data for 51 autosomal STRs and 17 Y-STRs included in the certificate of analysis.

Original languageEnglish
JournalForensic Science International: Genetics Supplement Series
Volume3
Issue number1
DOIs
StatePublished - 1 Dec 2011

Fingerprint

DNA Fingerprinting
Polymerase Chain Reaction
DNA
Cell Line

Keywords

  • Certified Reference Material (CRM), Standard Reference Material (SRM)
  • SRM 2391c
  • STR

Cite this

Kline, M. C. ; Butts, E. L.R. ; Hill, C. R. ; Coble, Michael Dewitt ; Duewer, D. L. ; Butler, J. M. / The new Standard Reference Material® 2391c : PCR-based DNA profiling standard. In: Forensic Science International: Genetics Supplement Series. 2011 ; Vol. 3, No. 1.
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The new Standard Reference Material® 2391c : PCR-based DNA profiling standard. / Kline, M. C.; Butts, E. L.R.; Hill, C. R.; Coble, Michael Dewitt; Duewer, D. L.; Butler, J. M.

In: Forensic Science International: Genetics Supplement Series, Vol. 3, No. 1, 01.12.2011.

Research output: Contribution to journalArticle

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