TY - JOUR
T1 - The lot-to-lot variability in the mitochondrial genome of controls
AU - Cihlar, Jennifer Churchill
AU - Peters, Dixie
AU - Strobl, Christina
AU - Parson, Walther
AU - Budowle, Bruce
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/7
Y1 - 2020/7
N2 - Current research in the biomedical field has illustrated how cell lines used as reference standards can change over time and, more importantly, can affect research and diagnostic results obtained from these cell lines. With the use of increasingly sensitive and highly resolving technologies (e.g., massively parallel sequencing), forensic scientists must be aware of and account for potential variability in the cell lines used as controls in their validation studies and day-to-day casework. In this study, multiple lot numbers from four commonly-used control cell line DNAs were sequenced with massively parallel sequencing on the Ion S5. The variability among these different lots was evaluated, and the effect on forensic laboratory work discussed.
AB - Current research in the biomedical field has illustrated how cell lines used as reference standards can change over time and, more importantly, can affect research and diagnostic results obtained from these cell lines. With the use of increasingly sensitive and highly resolving technologies (e.g., massively parallel sequencing), forensic scientists must be aware of and account for potential variability in the cell lines used as controls in their validation studies and day-to-day casework. In this study, multiple lot numbers from four commonly-used control cell line DNAs were sequenced with massively parallel sequencing on the Ion S5. The variability among these different lots was evaluated, and the effect on forensic laboratory work discussed.
KW - Control DNA
KW - Ion torrent
KW - Massively parallel sequencing
KW - Mitochondrial genome
UR - http://www.scopus.com/inward/record.url?scp=85084949053&partnerID=8YFLogxK
U2 - 10.1016/j.fsigen.2020.102298
DO - 10.1016/j.fsigen.2020.102298
M3 - Article
C2 - 32464353
AN - SCOPUS:85084949053
VL - 47
JO - Forensic Science International: Genetics
JF - Forensic Science International: Genetics
SN - 1872-4973
M1 - 102298
ER -