The lot-to-lot variability in the mitochondrial genome of controls

Jennifer Churchill Cihlar, Dixie Peters, Christina Strobl, Walther Parson, Bruce Budowle

Research output: Contribution to journalArticle

Abstract

Current research in the biomedical field has illustrated how cell lines used as reference standards can change over time and, more importantly, can affect research and diagnostic results obtained from these cell lines. With the use of increasingly sensitive and highly resolving technologies (e.g., massively parallel sequencing), forensic scientists must be aware of and account for potential variability in the cell lines used as controls in their validation studies and day-to-day casework. In this study, multiple lot numbers from four commonly-used control cell line DNAs were sequenced with massively parallel sequencing on the Ion S5. The variability among these different lots was evaluated, and the effect on forensic laboratory work discussed.

Original languageEnglish
Article number102298
JournalForensic Science International: Genetics
Volume47
DOIs
StatePublished - Jul 2020

Keywords

  • Control DNA
  • Ion torrent
  • Massively parallel sequencing
  • Mitochondrial genome

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