The inhibition of bovine pancreatic carboxypeptidase a by dihydroxyacetone phosphate

R. C. Adelman, Andras G. Lacko

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

In a recent study of the effects of various ligands on the rate of proteolytic digestion of rabbit muscle aldolase, it was observed that dihydroxyacetone phosphate (DHAP) acts as a powerful inhibitor of bovine pancreatic carboxypeptidase A (CPA) (Adelman et al., 1968). The present work demonstrates that this inhibition is competitive for both peptidase and esterase activities. Because this represents the first observation of competitive inhibition of CPA by a compound lacking an aromatic group, it was of interest to characterize further the structural requirements for this interaction.

Original languageEnglish
Pages (from-to)596-601
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume33
Issue number4
DOIs
StatePublished - 25 Nov 1968

Fingerprint

Dihydroxyacetone Phosphate
Carboxypeptidases A
Carboxypeptidases
Fructose-Bisphosphate Aldolase
Esterases
Muscle
Digestion
Peptide Hydrolases
Observation
Rabbits
Ligands
Muscles

Cite this

@article{2435228b034248e5ac2450df994c7810,
title = "The inhibition of bovine pancreatic carboxypeptidase a by dihydroxyacetone phosphate",
abstract = "In a recent study of the effects of various ligands on the rate of proteolytic digestion of rabbit muscle aldolase, it was observed that dihydroxyacetone phosphate (DHAP) acts as a powerful inhibitor of bovine pancreatic carboxypeptidase A (CPA) (Adelman et al., 1968). The present work demonstrates that this inhibition is competitive for both peptidase and esterase activities. Because this represents the first observation of competitive inhibition of CPA by a compound lacking an aromatic group, it was of interest to characterize further the structural requirements for this interaction.",
author = "Adelman, {R. C.} and Lacko, {Andras G.}",
year = "1968",
month = "11",
day = "25",
doi = "10.1016/0006-291X(68)90337-9",
language = "English",
volume = "33",
pages = "596--601",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

The inhibition of bovine pancreatic carboxypeptidase a by dihydroxyacetone phosphate. / Adelman, R. C.; Lacko, Andras G.

In: Biochemical and Biophysical Research Communications, Vol. 33, No. 4, 25.11.1968, p. 596-601.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The inhibition of bovine pancreatic carboxypeptidase a by dihydroxyacetone phosphate

AU - Adelman, R. C.

AU - Lacko, Andras G.

PY - 1968/11/25

Y1 - 1968/11/25

N2 - In a recent study of the effects of various ligands on the rate of proteolytic digestion of rabbit muscle aldolase, it was observed that dihydroxyacetone phosphate (DHAP) acts as a powerful inhibitor of bovine pancreatic carboxypeptidase A (CPA) (Adelman et al., 1968). The present work demonstrates that this inhibition is competitive for both peptidase and esterase activities. Because this represents the first observation of competitive inhibition of CPA by a compound lacking an aromatic group, it was of interest to characterize further the structural requirements for this interaction.

AB - In a recent study of the effects of various ligands on the rate of proteolytic digestion of rabbit muscle aldolase, it was observed that dihydroxyacetone phosphate (DHAP) acts as a powerful inhibitor of bovine pancreatic carboxypeptidase A (CPA) (Adelman et al., 1968). The present work demonstrates that this inhibition is competitive for both peptidase and esterase activities. Because this represents the first observation of competitive inhibition of CPA by a compound lacking an aromatic group, it was of interest to characterize further the structural requirements for this interaction.

UR - http://www.scopus.com/inward/record.url?scp=0014430261&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(68)90337-9

DO - 10.1016/0006-291X(68)90337-9

M3 - Article

VL - 33

SP - 596

EP - 601

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -