The genomic organisation and nucleotide sequence of the HLA-SB(DP) alpha gene

Simon K. Lawrance; Hriday K. Das; Julian Pan; Sherman M. Weissman

doi:10.1093/nar/13.20.7515

The genomic organisation and nucleotide sequence of the HLA-SB(DP) alpha gene

Simon K. Lawrance, Hriday K. Das, Julian Pan, Sherman M. Weissman

Institute for Healthy Aging

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Abstract

We have isolated a unique fragment of the HLA-DR alpha gene and probed human genomic DNA at low stringency to search for homologous sequences. A minimum of six non-polymorphic cross-hybridizing high molecular weight fragments were found in all DNAs examined. In order to obtain molecular clones of these cross-hybridizing fragments, we constructed lambda and cosmid libraries of human DNA and screened them at low stringency with the HLA-DR alpha gene specific subclone. We have isolated clones corresponding to each of the six fragments and, in this paper, describe those which contain the gene encoding HLA-SB(DP) alpha.

Original language	English
Pages (from-to)	7515-7528
Number of pages	14
Journal	Nucleic Acids Research
Volume	13
Issue number	20
DOIs	https://doi.org/10.1093/nar/13.20.7515
State	Published - 25 Oct 1985

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title = "The genomic organisation and nucleotide sequence of the HLA-SB(DP) alpha gene",

abstract = "We have isolated a unique fragment of the HLA-DR alpha gene and probed human genomic DNA at low stringency to search for homologous sequences. A minimum of six non-polymorphic cross-hybridizing high molecular weight fragments were found in all DNAs examined. In order to obtain molecular clones of these cross-hybridizing fragments, we constructed lambda and cosmid libraries of human DNA and screened them at low stringency with the HLA-DR alpha gene specific subclone. We have isolated clones corresponding to each of the six fragments and, in this paper, describe those which contain the gene encoding HLA-SB(DP) alpha.",

author = "Lawrance, {Simon K.} and Das, {Hriday K.} and Julian Pan and Weissman, {Sherman M.}",

note = "Funding Information: ACKNOWLEDGEMENTS The authors wish to acknowledge the excellent technical assistance of Ms. Julia Wang and Ms. Sherry Rucker and to thank Ms. Emily Lawrance for help in preparing the manuscript. We also thank Dr. Henry Erlich for providing the SB alpha cDNA sequence. This work was supported by National Institutes of Health Grants AM 33871-02.",

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doi = "10.1093/nar/13.20.7515",

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T1 - The genomic organisation and nucleotide sequence of the HLA-SB(DP) alpha gene

AU - Lawrance, Simon K.

AU - Das, Hriday K.

AU - Pan, Julian

AU - Weissman, Sherman M.

N1 - Funding Information: ACKNOWLEDGEMENTS The authors wish to acknowledge the excellent technical assistance of Ms. Julia Wang and Ms. Sherry Rucker and to thank Ms. Emily Lawrance for help in preparing the manuscript. We also thank Dr. Henry Erlich for providing the SB alpha cDNA sequence. This work was supported by National Institutes of Health Grants AM 33871-02.

PY - 1985/10/25

Y1 - 1985/10/25

N2 - We have isolated a unique fragment of the HLA-DR alpha gene and probed human genomic DNA at low stringency to search for homologous sequences. A minimum of six non-polymorphic cross-hybridizing high molecular weight fragments were found in all DNAs examined. In order to obtain molecular clones of these cross-hybridizing fragments, we constructed lambda and cosmid libraries of human DNA and screened them at low stringency with the HLA-DR alpha gene specific subclone. We have isolated clones corresponding to each of the six fragments and, in this paper, describe those which contain the gene encoding HLA-SB(DP) alpha.

AB - We have isolated a unique fragment of the HLA-DR alpha gene and probed human genomic DNA at low stringency to search for homologous sequences. A minimum of six non-polymorphic cross-hybridizing high molecular weight fragments were found in all DNAs examined. In order to obtain molecular clones of these cross-hybridizing fragments, we constructed lambda and cosmid libraries of human DNA and screened them at low stringency with the HLA-DR alpha gene specific subclone. We have isolated clones corresponding to each of the six fragments and, in this paper, describe those which contain the gene encoding HLA-SB(DP) alpha.

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The genomic organisation and nucleotide sequence of the HLA-SB(DP) alpha gene

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