The genomic organisation and nucleotide sequence of the HLA-SB(DP) alpha gene

Simon K. Lawrance, Hriday K. Das, Julian Pan, Sherman M. Weissman

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We have isolated a unique fragment of the HLA-DR alpha gene and probed human genomic DNA at low stringency to search for homologous sequences. A minimum of six non-polymorphic cross-hybridizing high molecular weight fragments were found in all DNAs examined. In order to obtain molecular clones of these cross-hybridizing fragments, we constructed lambda and cosmid libraries of human DNA and screened them at low stringency with the HLA-DR alpha gene specific subclone. We have isolated clones corresponding to each of the six fragments and, in this paper, describe those which contain the gene encoding HLA-SB(DP) alpha.

Original languageEnglish
Pages (from-to)7515-7528
Number of pages14
JournalNucleic Acids Research
Volume13
Issue number20
DOIs
StatePublished - 25 Oct 1985

Fingerprint

HLA-DP Antigens
HLA-DR alpha-Chains
Clone Cells
Genes
Cosmids
DNA
Sequence Homology
Gene Library
Molecular Weight

Cite this

Lawrance, Simon K. ; Das, Hriday K. ; Pan, Julian ; Weissman, Sherman M. / The genomic organisation and nucleotide sequence of the HLA-SB(DP) alpha gene. In: Nucleic Acids Research. 1985 ; Vol. 13, No. 20. pp. 7515-7528.
@article{270e2d53873a41dfafc742e95a725941,
title = "The genomic organisation and nucleotide sequence of the HLA-SB(DP) alpha gene",
abstract = "We have isolated a unique fragment of the HLA-DR alpha gene and probed human genomic DNA at low stringency to search for homologous sequences. A minimum of six non-polymorphic cross-hybridizing high molecular weight fragments were found in all DNAs examined. In order to obtain molecular clones of these cross-hybridizing fragments, we constructed lambda and cosmid libraries of human DNA and screened them at low stringency with the HLA-DR alpha gene specific subclone. We have isolated clones corresponding to each of the six fragments and, in this paper, describe those which contain the gene encoding HLA-SB(DP) alpha.",
author = "Lawrance, {Simon K.} and Das, {Hriday K.} and Julian Pan and Weissman, {Sherman M.}",
year = "1985",
month = "10",
day = "25",
doi = "10.1093/nar/13.20.7515",
language = "English",
volume = "13",
pages = "7515--7528",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "20",

}

The genomic organisation and nucleotide sequence of the HLA-SB(DP) alpha gene. / Lawrance, Simon K.; Das, Hriday K.; Pan, Julian; Weissman, Sherman M.

In: Nucleic Acids Research, Vol. 13, No. 20, 25.10.1985, p. 7515-7528.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The genomic organisation and nucleotide sequence of the HLA-SB(DP) alpha gene

AU - Lawrance, Simon K.

AU - Das, Hriday K.

AU - Pan, Julian

AU - Weissman, Sherman M.

PY - 1985/10/25

Y1 - 1985/10/25

N2 - We have isolated a unique fragment of the HLA-DR alpha gene and probed human genomic DNA at low stringency to search for homologous sequences. A minimum of six non-polymorphic cross-hybridizing high molecular weight fragments were found in all DNAs examined. In order to obtain molecular clones of these cross-hybridizing fragments, we constructed lambda and cosmid libraries of human DNA and screened them at low stringency with the HLA-DR alpha gene specific subclone. We have isolated clones corresponding to each of the six fragments and, in this paper, describe those which contain the gene encoding HLA-SB(DP) alpha.

AB - We have isolated a unique fragment of the HLA-DR alpha gene and probed human genomic DNA at low stringency to search for homologous sequences. A minimum of six non-polymorphic cross-hybridizing high molecular weight fragments were found in all DNAs examined. In order to obtain molecular clones of these cross-hybridizing fragments, we constructed lambda and cosmid libraries of human DNA and screened them at low stringency with the HLA-DR alpha gene specific subclone. We have isolated clones corresponding to each of the six fragments and, in this paper, describe those which contain the gene encoding HLA-SB(DP) alpha.

UR - http://www.scopus.com/inward/record.url?scp=0022432458&partnerID=8YFLogxK

U2 - 10.1093/nar/13.20.7515

DO - 10.1093/nar/13.20.7515

M3 - Article

C2 - 2997750

AN - SCOPUS:0022432458

VL - 13

SP - 7515

EP - 7528

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 20

ER -