Met-RANTES is a modified version of the chemokine RANTES, which has an additional amino acid at the amino terminus. To compare Met-RANTES with the native protein as well as with another antagonist, AOP-RANTES, we have solved the structure of Met-RANTES at 1.6 Å by X-ray crystallography and investigated the dimerization of all three proteins by fluorescence anisotropy. The dimerization of AOP-RANTES shows an altered aggregation profile. The delineation of slight differences in binding sulfate anions by Met- and AOP-RANTES might be linked to specific yet different interactions between these proteins and glycosaminoglycans.
|Number of pages||10|
|Journal||Protein and Peptide Letters|
|State||Published - 1 Dec 2000|