The crystal structure of Met-RANTES: Comparison with native RANTES and AOP-RANTES

David M. Hoover; Jeffrey Shaw; Zygmunt Gryczynski; Amanda E.I. Proudfoot; Tim Wells; Jacek Lubkowski

The crystal structure of Met-RANTES: Comparison with native RANTES and AOP-RANTES

David M. Hoover, Jeffrey Shaw, Zygmunt Gryczynski, Amanda E.I. Proudfoot, Tim Wells, Jacek Lubkowski

Research output: Contribution to journal › Article › peer-review

Abstract

Met-RANTES is a modified version of the chemokine RANTES, which has an additional amino acid at the amino terminus. To compare Met-RANTES with the native protein as well as with another antagonist, AOP-RANTES, we have solved the structure of Met-RANTES at 1.6 Å by X-ray crystallography and investigated the dimerization of all three proteins by fluorescence anisotropy. The dimerization of AOP-RANTES shows an altered aggregation profile. The delineation of slight differences in binding sulfate anions by Met- and AOP-RANTES might be linked to specific yet different interactions between these proteins and glycosaminoglycans.

Original language	English
Pages (from-to)	73-82
Number of pages	10
Journal	Protein and Peptide Letters
Volume	7
Issue number	2
State	Published - 1 Dec 2000

Cite this

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title = "The crystal structure of Met-RANTES: Comparison with native RANTES and AOP-RANTES",

abstract = "Met-RANTES is a modified version of the chemokine RANTES, which has an additional amino acid at the amino terminus. To compare Met-RANTES with the native protein as well as with another antagonist, AOP-RANTES, we have solved the structure of Met-RANTES at 1.6 {\AA} by X-ray crystallography and investigated the dimerization of all three proteins by fluorescence anisotropy. The dimerization of AOP-RANTES shows an altered aggregation profile. The delineation of slight differences in binding sulfate anions by Met- and AOP-RANTES might be linked to specific yet different interactions between these proteins and glycosaminoglycans.",

author = "Hoover, {David M.} and Jeffrey Shaw and Zygmunt Gryczynski and Proudfoot, {Amanda E.I.} and Tim Wells and Jacek Lubkowski",

year = "2000",

month = dec,

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language = "English",

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TY - JOUR

T1 - The crystal structure of Met-RANTES

T2 - Comparison with native RANTES and AOP-RANTES

AU - Hoover, David M.

AU - Shaw, Jeffrey

AU - Gryczynski, Zygmunt

AU - Proudfoot, Amanda E.I.

AU - Wells, Tim

AU - Lubkowski, Jacek

PY - 2000/12/1

Y1 - 2000/12/1

N2 - Met-RANTES is a modified version of the chemokine RANTES, which has an additional amino acid at the amino terminus. To compare Met-RANTES with the native protein as well as with another antagonist, AOP-RANTES, we have solved the structure of Met-RANTES at 1.6 Å by X-ray crystallography and investigated the dimerization of all three proteins by fluorescence anisotropy. The dimerization of AOP-RANTES shows an altered aggregation profile. The delineation of slight differences in binding sulfate anions by Met- and AOP-RANTES might be linked to specific yet different interactions between these proteins and glycosaminoglycans.

AB - Met-RANTES is a modified version of the chemokine RANTES, which has an additional amino acid at the amino terminus. To compare Met-RANTES with the native protein as well as with another antagonist, AOP-RANTES, we have solved the structure of Met-RANTES at 1.6 Å by X-ray crystallography and investigated the dimerization of all three proteins by fluorescence anisotropy. The dimerization of AOP-RANTES shows an altered aggregation profile. The delineation of slight differences in binding sulfate anions by Met- and AOP-RANTES might be linked to specific yet different interactions between these proteins and glycosaminoglycans.

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M3 - Article

AN - SCOPUS:0002089788

SN - 0929-8665

VL - 7

SP - 73

EP - 82

JO - Protein and Peptide Letters

JF - Protein and Peptide Letters

IS - 2

ER -

The crystal structure of Met-RANTES: Comparison with native RANTES and AOP-RANTES

Abstract

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