The construction of leukaryotic expression plasmid PCDNA3/HPV16E6 and experimental observation of naked DNA injected into animal

X. Youyuan, W. Yili, S. Lusheng, S. Xiangle

Research output: Contribution to journalArticle

Abstract

HPV16 infection is closely associated with cervical carcinomas. A lot of investigation indicate that HPV16 E6/E7 proteins can act as tumor rejection antigens. So an HPV16E6 leukaryotic expression plasmid pcDNA3/E6 was constructed and transfected into Cos7 cells, which expressed E6 protein in vitro. Animal experiments proved that intramuscular immunization with plasmid pcDNA3/E6 could elicit specific immunoresponse against HPV16 E6 in mice. All these results suggest that E6 DNA vaccine can serve as a candidate therapeutic vaccine for HPV16 associated diseases in humans.

Original languageEnglish
Pages (from-to)115-119
Number of pages5
JournalJournal of Xi'an Medical University, English Edition
Volume10
Issue number2
StatePublished - 1 Dec 1998

Keywords

  • Cervical cancer
  • DNA vaccine
  • HPV16E6

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