Abstract
Infection with high risk human papillomavirus is regarded as the major risk factor in the development of cervical cancer. In this study, HPV16-L1 eukaryotic expression plasmids pcDNA-L1 were constructed, which were transfected into mammalian cells Cos-7. The expression of HPV16-L1 in transfected cells were identified by in situ hybridization, immunospot and immunocytochemistry. HPV16-L1 mRNA transcription and L1 protein expression were found in recombinant plasmid transfected cells. This expression system will provide us with plentiful resource for HPV16-L1 immunological study and will be helpful for the design of HPV16 prophylactic vaccine.
Original language | English |
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Pages (from-to) | 116-119 |
Number of pages | 4 |
Journal | Journal of Xi'an Medical University, English Edition |
Volume | 11 |
Issue number | 2 |
State | Published - 1999 |
Keywords
- Cervical carcinoma
- Eukaryotic expression
- Human papillomavirus type 16(HPV16-L1)