Infection with high risk human papillomavirus is regarded as the major risk factor in the development of cervical cancer. In this study, HPV16-L1 eukaryotic expression plasmids pcDNA-L1 were constructed, which were transfected into mammalian cells Cos-7. The expression of HPV16-L1 in transfected cells were identified by in situ hybridization, immunospot and immunocytochemistry. HPV16-L1 mRNA transcription and L1 protein expression were found in recombinant plasmid transfected cells. This expression system will provide us with plentiful resource for HPV16-L1 immunological study and will be helpful for the design of HPV16 prophylactic vaccine.
|Number of pages||4|
|Journal||Journal of Xi'an Medical University, English Edition|
|State||Published - 1 Dec 1999|
- Cervical carcinoma
- Eukaryotic expression
- Human papillomavirus type 16(HPV16-L1)