The construction of HPV16-L1 recombinant plasmid and its expression in mammalian cells

Infection with high risk human papillomavirus is regarded as the major risk factor in the development of cervical cancer. In this study, HPV16-L1 eukaryotic expression plasmids pcDNA-L1 were constructed, which were transfected into mammalian cells Cos-7. The expression of HPV16-L1 in transfected cells were identified by in situ hybridization, immunospot and immunocytochemistry. HPV16-L1 mRNA transcription and L1 protein expression were found in recombinant plasmid transfected cells. This expression system will provide us with plentiful resource for HPV16-L1 immunological study and will be helpful for the design of HPV16 prophylactic vaccine.