The construction of HPV16-L1 recombinant plasmid and its expression in mammalian cells

Xiang Le Sun, L. Si, Z. Cao, Y. Wang, T. Liu, J. Guo, J. Song

Research output: Contribution to journalArticle

Abstract

Infection with high risk human papillomavirus is regarded as the major risk factor in the development of cervical cancer. In this study, HPV16-L1 eukaryotic expression plasmids pcDNA-L1 were constructed, which were transfected into mammalian cells Cos-7. The expression of HPV16-L1 in transfected cells were identified by in situ hybridization, immunospot and immunocytochemistry. HPV16-L1 mRNA transcription and L1 protein expression were found in recombinant plasmid transfected cells. This expression system will provide us with plentiful resource for HPV16-L1 immunological study and will be helpful for the design of HPV16 prophylactic vaccine.

Original languageEnglish
Pages (from-to)116-119
Number of pages4
JournalJournal of Xi'an Medical University, English Edition
Volume11
Issue number2
StatePublished - 1 Dec 1999

Keywords

  • Cervical carcinoma
  • Eukaryotic expression
  • Human papillomavirus type 16(HPV16-L1)

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    Sun, X. L., Si, L., Cao, Z., Wang, Y., Liu, T., Guo, J., & Song, J. (1999). The construction of HPV16-L1 recombinant plasmid and its expression in mammalian cells. Journal of Xi'an Medical University, English Edition, 11(2), 116-119.