TY - JOUR
T1 - The antidepressant effects of curcumin in the forced swimming test involve 5-HT1 and 5-HT2 receptors
AU - Wang, Rui
AU - Xu, Ying
AU - Wu, Hong Li
AU - Li, Ying Bo
AU - Li, Yu Hua
AU - Guo, Jia Bin
AU - Li, Xue Jun
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (No. 30171090, 30270528, 30572202, 30772571), 973 Program of the Ministry of Science and Technology in China (No. 2004CB518902). The research fund comes from Ministry of Education of China No. 20020001082 as well as 985 and 211 Projects of Ministry of Education of China.
PY - 2008/1/6
Y1 - 2008/1/6
N2 - Curcuma longa is a main constituent of many traditional Chinese medicines, such as Xiaoyao-san, used to manage mental disorders effectively. Curcumin is a major active component of C. longa and its antidepressant-like effect has been previously demonstrated in the forced swimming test. The purpose of this study was to explore the possible contribution of serotonin (5-HT) receptors in the behavioral effects induced by curcumin in this animal model of depression. 5-HT was depleted by the tryptophan hydroxylase inhibitor p-chlorophenylalanine (PCPA, 100 mg/kg, i.p.) prior to the administration of curcumin, and the consequent results showed that PCPA blocked the anti-immobility effect of curcumin in forced swimming test, suggesting the involvement of the serotonergic system. Moreover, pre-treatment of pindolol (10 mg/kg, i.p., a β-adrenoceptors blocker/5-HT1A/1B receptor antagonist), 4-(2′-methoxy-phenyl)-1-[2′-(n-2″-pyridinyl)-p-iodobenzamino-]ethyl-piperazine (p-MPPI, 1 mg/kg, s.c., a selective 5-HT1A receptor antagonist), or 1-(2-(1-pyrrolyl)-phenoxy)-3-isopropylamino-2-propanol (isamoltane, 2.5 mg/kg, i.p., a 5-HT1B receptor antagonist) was found to prevent the effect of curcumin (10 mg/kg) in forced swimming test. On the other hand, a sub-effective dose of curcumin (2.5 mg/kg, p.o.) produced a synergistic effect when given jointly with (+)-8-hydroxy-2-(di-n-propylamino)tetralin, (8-OH-DPAT, 1 mg/kg, i.p., a 5-HT1A receptor agonist), anpirtoline (0.25 mg/kg, i.p., a 5-HT1B receptor agonist) or ritanserin (4 mg/kg, i.p., a 5-HT2A/2C receptor antagonist), but not with ketanserin (5 mg/kg, i.p., a 5-HT2A/2C receptor antagonist with higher affinity to 5-HT2A receptor) or R(-)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI, 1 mg/kg, i.p., a 5-HT2A receptor agonist). Taken together, these results indicate that the antidepressant-like effect of curcumin in the forced swimming test is related to serotonergic system and may be mediated by, at least in part, an interaction with 5-HT1A/1B and 5-HT2C receptors.
AB - Curcuma longa is a main constituent of many traditional Chinese medicines, such as Xiaoyao-san, used to manage mental disorders effectively. Curcumin is a major active component of C. longa and its antidepressant-like effect has been previously demonstrated in the forced swimming test. The purpose of this study was to explore the possible contribution of serotonin (5-HT) receptors in the behavioral effects induced by curcumin in this animal model of depression. 5-HT was depleted by the tryptophan hydroxylase inhibitor p-chlorophenylalanine (PCPA, 100 mg/kg, i.p.) prior to the administration of curcumin, and the consequent results showed that PCPA blocked the anti-immobility effect of curcumin in forced swimming test, suggesting the involvement of the serotonergic system. Moreover, pre-treatment of pindolol (10 mg/kg, i.p., a β-adrenoceptors blocker/5-HT1A/1B receptor antagonist), 4-(2′-methoxy-phenyl)-1-[2′-(n-2″-pyridinyl)-p-iodobenzamino-]ethyl-piperazine (p-MPPI, 1 mg/kg, s.c., a selective 5-HT1A receptor antagonist), or 1-(2-(1-pyrrolyl)-phenoxy)-3-isopropylamino-2-propanol (isamoltane, 2.5 mg/kg, i.p., a 5-HT1B receptor antagonist) was found to prevent the effect of curcumin (10 mg/kg) in forced swimming test. On the other hand, a sub-effective dose of curcumin (2.5 mg/kg, p.o.) produced a synergistic effect when given jointly with (+)-8-hydroxy-2-(di-n-propylamino)tetralin, (8-OH-DPAT, 1 mg/kg, i.p., a 5-HT1A receptor agonist), anpirtoline (0.25 mg/kg, i.p., a 5-HT1B receptor agonist) or ritanserin (4 mg/kg, i.p., a 5-HT2A/2C receptor antagonist), but not with ketanserin (5 mg/kg, i.p., a 5-HT2A/2C receptor antagonist with higher affinity to 5-HT2A receptor) or R(-)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI, 1 mg/kg, i.p., a 5-HT2A receptor agonist). Taken together, these results indicate that the antidepressant-like effect of curcumin in the forced swimming test is related to serotonergic system and may be mediated by, at least in part, an interaction with 5-HT1A/1B and 5-HT2C receptors.
KW - 5-HT receptors
KW - 5-HT receptors
KW - Antidepressant
KW - Curcumin
KW - Depression
KW - Forced swimming test
UR - http://www.scopus.com/inward/record.url?scp=36549008159&partnerID=8YFLogxK
U2 - 10.1016/j.ejphar.2007.08.045
DO - 10.1016/j.ejphar.2007.08.045
M3 - Article
C2 - 17942093
AN - SCOPUS:36549008159
SN - 0014-2999
VL - 578
SP - 43
EP - 50
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1
ER -