The analysis of EDTA in dried bloodstains by electrospray LC-MS-MS and ion chromatography

Mark L. Miller; Bruce R. McCord; Roger Martz; Bruce Budowle

doi:10.1093/jat/21.7.521

The analysis of EDTA in dried bloodstains by electrospray LC-MS-MS and ion chromatography

Mark L. Miller, Bruce R. McCord, Roger Martz, Bruce Budowle

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15 Scopus citations

Abstract

Analytical methods were developed to determine the presence of ethylenedlaminetetraacetic acid (EDTA) in dried bloodstains to provide probative information when allegations of evidence tampering have been made in criminal cases. A simple screening method using ion chromatography to analyze stains was found to be quantitative to the 5 ppm level. The presence of EDTA was then confirmed using negative and positive ion mode liquid chromatography-tandem mass spectrometry (LC-MS-MS) methods. A blind trial of these methods on 42 samples correctly determined the bloodstains that did and did not contain the preservative EDTA. One interesting observation in these results was the adsorption and postanalysis release of EDTA in the chromatographic system. In order to avoid cross contamination of samples resulting from this phenomena, it was found to be necessary to use EDTA-free blood extracts as blanks in the LC-MS analysis of bloodstains.

Original language	English
Pages (from-to)	521-528
Number of pages	8
Journal	Journal of Analytical Toxicology
Volume	21
Issue number	7
DOIs	https://doi.org/10.1093/jat/21.7.521
State	Published - 1997

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title = "The analysis of EDTA in dried bloodstains by electrospray LC-MS-MS and ion chromatography",

abstract = "Analytical methods were developed to determine the presence of ethylenedlaminetetraacetic acid (EDTA) in dried bloodstains to provide probative information when allegations of evidence tampering have been made in criminal cases. A simple screening method using ion chromatography to analyze stains was found to be quantitative to the 5 ppm level. The presence of EDTA was then confirmed using negative and positive ion mode liquid chromatography-tandem mass spectrometry (LC-MS-MS) methods. A blind trial of these methods on 42 samples correctly determined the bloodstains that did and did not contain the preservative EDTA. One interesting observation in these results was the adsorption and postanalysis release of EDTA in the chromatographic system. In order to avoid cross contamination of samples resulting from this phenomena, it was found to be necessary to use EDTA-free blood extracts as blanks in the LC-MS analysis of bloodstains.",

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AU - Miller, Mark L.

AU - McCord, Bruce R.

AU - Martz, Roger

AU - Budowle, Bruce

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AB - Analytical methods were developed to determine the presence of ethylenedlaminetetraacetic acid (EDTA) in dried bloodstains to provide probative information when allegations of evidence tampering have been made in criminal cases. A simple screening method using ion chromatography to analyze stains was found to be quantitative to the 5 ppm level. The presence of EDTA was then confirmed using negative and positive ion mode liquid chromatography-tandem mass spectrometry (LC-MS-MS) methods. A blind trial of these methods on 42 samples correctly determined the bloodstains that did and did not contain the preservative EDTA. One interesting observation in these results was the adsorption and postanalysis release of EDTA in the chromatographic system. In order to avoid cross contamination of samples resulting from this phenomena, it was found to be necessary to use EDTA-free blood extracts as blanks in the LC-MS analysis of bloodstains.

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The analysis of EDTA in dried bloodstains by electrospray LC-MS-MS and ion chromatography

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