The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis

Zhicheng Shao; Ruowen Zhang; Alireza Khodadadi-Jamayran; Bo Chen; Michael R. Crowley; Muhamad A. Festok; David K. Crossman; Tim M. Townes; Kejin Hu

doi:10.1038/ncomms10869

The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis

Zhicheng Shao, Ruowen Zhang, Alireza Khodadadi-Jamayran, Bo Chen, Michael R. Crowley, Muhamad A. Festok, David K. Crossman, Tim M. Townes, Kejin Hu

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19 Scopus citations

Abstract

It is well known that both recipient cells and donor nuclei demonstrate a mitotic advantage as observed in the traditional reprogramming with somatic cell nuclear transfer (SCNT). However, it is not known whether a specific mitotic factor plays a critical role in reprogramming. Here we identify an isoform of human bromodomain-containing 3 (BRD3), BRD3R (BRD3 with Reprogramming activity), as a reprogramming factor. BRD3R positively regulates mitosis during reprogramming, upregulates a large set of mitotic genes at early stages of reprogramming, and associates with mitotic chromatin. Interestingly, a set of the mitotic genes upregulated by BRD3R constitutes a pluripotent molecular signature. The two BRD3 isoforms display differential binding to acetylated histones. Our results suggest a molecular interpretation for the mitotic advantage in reprogramming and show that mitosis may be a driving force of reprogramming.

Original language	English
Article number	10869
Journal	Nature Communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms10869
State	Published - 7 Mar 2016

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title = "The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis",

abstract = "It is well known that both recipient cells and donor nuclei demonstrate a mitotic advantage as observed in the traditional reprogramming with somatic cell nuclear transfer (SCNT). However, it is not known whether a specific mitotic factor plays a critical role in reprogramming. Here we identify an isoform of human bromodomain-containing 3 (BRD3), BRD3R (BRD3 with Reprogramming activity), as a reprogramming factor. BRD3R positively regulates mitosis during reprogramming, upregulates a large set of mitotic genes at early stages of reprogramming, and associates with mitotic chromatin. Interestingly, a set of the mitotic genes upregulated by BRD3R constitutes a pluripotent molecular signature. The two BRD3 isoforms display differential binding to acetylated histones. Our results suggest a molecular interpretation for the mitotic advantage in reprogramming and show that mitosis may be a driving force of reprogramming.",

author = "Zhicheng Shao and Ruowen Zhang and Alireza Khodadadi-Jamayran and Bo Chen and Crowley, {Michael R.} and Festok, {Muhamad A.} and Crossman, {David K.} and Townes, {Tim M.} and Kejin Hu",

note = "Funding Information: This research was supported by a UAB new faculty setup fund to KH, and in part by a seed grant from Alabama Institute of Medicine (AIMG1003) to KH. We thank Shawn Williams for his technical help in confocal imaging. We appreciate discussions from colleagues at UAB Stem Cell Institute during this research. We thank Drs Louise T. Chow and Kevin Pawlik for their critical reading.",

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doi = "10.1038/ncomms10869",

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AU - Shao, Zhicheng

AU - Zhang, Ruowen

AU - Khodadadi-Jamayran, Alireza

AU - Chen, Bo

AU - Crowley, Michael R.

AU - Festok, Muhamad A.

AU - Crossman, David K.

AU - Townes, Tim M.

AU - Hu, Kejin

N1 - Funding Information: This research was supported by a UAB new faculty setup fund to KH, and in part by a seed grant from Alabama Institute of Medicine (AIMG1003) to KH. We thank Shawn Williams for his technical help in confocal imaging. We appreciate discussions from colleagues at UAB Stem Cell Institute during this research. We thank Drs Louise T. Chow and Kevin Pawlik for their critical reading.

PY - 2016/3/7

Y1 - 2016/3/7

N2 - It is well known that both recipient cells and donor nuclei demonstrate a mitotic advantage as observed in the traditional reprogramming with somatic cell nuclear transfer (SCNT). However, it is not known whether a specific mitotic factor plays a critical role in reprogramming. Here we identify an isoform of human bromodomain-containing 3 (BRD3), BRD3R (BRD3 with Reprogramming activity), as a reprogramming factor. BRD3R positively regulates mitosis during reprogramming, upregulates a large set of mitotic genes at early stages of reprogramming, and associates with mitotic chromatin. Interestingly, a set of the mitotic genes upregulated by BRD3R constitutes a pluripotent molecular signature. The two BRD3 isoforms display differential binding to acetylated histones. Our results suggest a molecular interpretation for the mitotic advantage in reprogramming and show that mitosis may be a driving force of reprogramming.

AB - It is well known that both recipient cells and donor nuclei demonstrate a mitotic advantage as observed in the traditional reprogramming with somatic cell nuclear transfer (SCNT). However, it is not known whether a specific mitotic factor plays a critical role in reprogramming. Here we identify an isoform of human bromodomain-containing 3 (BRD3), BRD3R (BRD3 with Reprogramming activity), as a reprogramming factor. BRD3R positively regulates mitosis during reprogramming, upregulates a large set of mitotic genes at early stages of reprogramming, and associates with mitotic chromatin. Interestingly, a set of the mitotic genes upregulated by BRD3R constitutes a pluripotent molecular signature. The two BRD3 isoforms display differential binding to acetylated histones. Our results suggest a molecular interpretation for the mitotic advantage in reprogramming and show that mitosis may be a driving force of reprogramming.

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SN - 2041-1723

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The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis

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