TY - JOUR
T1 - Tetracycline-containing MCM-41 mesoporous silica nanoparticles for the treatment of Escherichia coli
AU - Koneru, Bhuvaneswari
AU - Shi, Yi
AU - Wang, Yu Chieh
AU - Chavala, Sai H.
AU - Miller, Michael L.
AU - Holbert, Brittany
AU - Conson, Maricar
AU - Ni, Aiguo
AU - Di Pasqua, Anthony J.
N1 - Publisher Copyright:
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
PY - 2015/10/30
Y1 - 2015/10/30
N2 - Tetracycline (TC) is a well-known broad spectrum antibiotic, which is effective against many Gram positive and Gram negative bacteria. Controlled release nanoparticle formulations of TC have been reported, and could be beneficial for application in the treatment of periodontitis and dental bone infections. Furthermore, TC-controlled transcriptional regulation systems (Tet-on and Tet-off) are useful for controlling transgene expression in vitro and in vivo for biomedical research purposes; controlled TC release systems could be useful here, as well. Mesoporous silica nanomaterials (MSNs) are widely studied for drug delivery applications; Mobile crystalline material 41 (MCM-41), a type of MSN, has a mesoporous structure with pores forming channels in a hexagonal fashion. We prepared 41 4 and 406 55 nm MCM-41 mesoporous silica nanoparticles with loaded TC for controlled drug release; TC content in the TC-MCM-41 nanoparticles was 18.7% and 17.7% w/w, respectively. Release of TC from TC-MCM-41 nanoparticles was then measured in phosphate-buffered saline (PBS), pH 7.2, at 37 C over a period of 5 h. Most antibiotic was released from both over this observation period; however, the majority of TC was released over the first hour. Efficacy of the TC-MCM-41 nanoparticles was then shown to be superior to free TC against Escherichia coli (E. coli) in culture over a 24 h period, while blank nanoparticles had no effect.
AB - Tetracycline (TC) is a well-known broad spectrum antibiotic, which is effective against many Gram positive and Gram negative bacteria. Controlled release nanoparticle formulations of TC have been reported, and could be beneficial for application in the treatment of periodontitis and dental bone infections. Furthermore, TC-controlled transcriptional regulation systems (Tet-on and Tet-off) are useful for controlling transgene expression in vitro and in vivo for biomedical research purposes; controlled TC release systems could be useful here, as well. Mesoporous silica nanomaterials (MSNs) are widely studied for drug delivery applications; Mobile crystalline material 41 (MCM-41), a type of MSN, has a mesoporous structure with pores forming channels in a hexagonal fashion. We prepared 41 4 and 406 55 nm MCM-41 mesoporous silica nanoparticles with loaded TC for controlled drug release; TC content in the TC-MCM-41 nanoparticles was 18.7% and 17.7% w/w, respectively. Release of TC from TC-MCM-41 nanoparticles was then measured in phosphate-buffered saline (PBS), pH 7.2, at 37 C over a period of 5 h. Most antibiotic was released from both over this observation period; however, the majority of TC was released over the first hour. Efficacy of the TC-MCM-41 nanoparticles was then shown to be superior to free TC against Escherichia coli (E. coli) in culture over a 24 h period, while blank nanoparticles had no effect.
KW - Controlled drug release
KW - E. coli
KW - MCM-41
KW - Tetracycline
UR - http://www.scopus.com/inward/record.url?scp=84949948608&partnerID=8YFLogxK
U2 - 10.3390/molecules201119650
DO - 10.3390/molecules201119650
M3 - Article
C2 - 26528964
AN - SCOPUS:84949948608
SN - 1420-3049
VL - 20
SP - 19690
EP - 19698
JO - Molecules
JF - Molecules
IS - 11
ER -