Abstract
The technique for mechanical dissection of microscopic myofibrilar bundles containing few myofibrils is described. A sensitive electrooptical tension transducer capable of resolving the tension generated by such myofibrilar bundles to within a fraction of a percent is used to study the response of the preparations to low ATP concentrations. The rate of tension development decreased with a decrease in ATP concentration. Below 10-6 M ATP the myofibrils did not demonstrate any mechanical activity at all. These findings are discussed in terms of the kinetics of the crossbridge cycle.
| Original language | English |
|---|---|
| Pages (from-to) | 189-204 |
| Number of pages | 16 |
| Journal | Journal of Mechanochemistry and Cell Motility |
| Volume | 4 |
| Issue number | 3 |
| State | Published - 1 Dec 1977 |
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Tension generation by isolated myofibrils. / Borejdo, Julian; Schweitzer, A.
In: Journal of Mechanochemistry and Cell Motility, Vol. 4, No. 3, 01.12.1977, p. 189-204.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Tension generation by isolated myofibrils
AU - Borejdo, Julian
AU - Schweitzer, A.
PY - 1977/12/1
Y1 - 1977/12/1
N2 - The technique for mechanical dissection of microscopic myofibrilar bundles containing few myofibrils is described. A sensitive electrooptical tension transducer capable of resolving the tension generated by such myofibrilar bundles to within a fraction of a percent is used to study the response of the preparations to low ATP concentrations. The rate of tension development decreased with a decrease in ATP concentration. Below 10-6 M ATP the myofibrils did not demonstrate any mechanical activity at all. These findings are discussed in terms of the kinetics of the crossbridge cycle.
AB - The technique for mechanical dissection of microscopic myofibrilar bundles containing few myofibrils is described. A sensitive electrooptical tension transducer capable of resolving the tension generated by such myofibrilar bundles to within a fraction of a percent is used to study the response of the preparations to low ATP concentrations. The rate of tension development decreased with a decrease in ATP concentration. Below 10-6 M ATP the myofibrils did not demonstrate any mechanical activity at all. These findings are discussed in terms of the kinetics of the crossbridge cycle.
UR - http://www.scopus.com/inward/record.url?scp=0017607575&partnerID=8YFLogxK
M3 - Article
VL - 4
SP - 189
EP - 204
JO - Journal of Mechanochemistry and Cell Motility
JF - Journal of Mechanochemistry and Cell Motility
IS - 3
ER -