Tandem sulfur-containing amino acids are epicritical determinants of dopamine D2 receptor pharmacology

John A. Schetz, David R. Sibley

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The conserved aspartic acid that is required for ligand binding to the dopamine D2 receptor is followed by three tandem sulfur-containing amino acids. While previous point mutation studies did not reveal any single one of these residues as being critical for ligand binding, we now show that simultaneously substituting all three with isovolumetric, non sulfur-containing amino acids results in large decreases in the binding affinity for dopamine, (-)-raclopride and 7-(-4(4-(2,3-dichlorophenyl)-1-piperazinyl)butyloxy)-3,4-dihydro-2(1H)-quinolinone (aripiprazole), but not for methylspiperone or allosteric modulators.

Original languageEnglish
Pages (from-to)R5-R7
JournalEuropean Journal of Pharmacology
Volume388
Issue number2
DOIs
StatePublished - 28 Jan 2000

Keywords

  • Allosteric modulator
  • Catecholamine
  • Mutagenesis

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